Raman spectroscopic of osteoporosis model in mouse tibia in vivo

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dc.creatorSamuel Monzem
dc.creatorDábila Araújo Sônego
dc.creatorAndresa de Cássia Martini
dc.creatorAna Paula Bispo Dantas Moura
dc.creatorFelipe Gomes da Silva
dc.creatorJorge Luiz Brito de Faria
dc.creatorRoberto Lopes de Souza
dc.date.accessioned2023-02-02T12:58:32Z
dc.date.accessioned2025-09-08T23:16:34Z
dc.date.available2023-02-02T12:58:32Z
dc.date.issued2018-09
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1016/j.vibspec.2018.07.013
dc.identifier.issn1873-3697
dc.identifier.urihttps://hdl.handle.net/1843/49430
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofVibrational Spectroscopy
dc.rightsAcesso Restrito
dc.subjectRaman, Espectroscopia de
dc.subjectHidroxiapatita
dc.subjectOsteoporose
dc.subjectTíbia
dc.titleRaman spectroscopic of osteoporosis model in mouse tibia in vivo
dc.typeArtigo de periódico
local.citation.epage91
local.citation.spage88
local.citation.volume98
local.description.resumoIn this work, we correlate Raman spectroscopy in vivo which is performed in healthy bone tibia, 3-month-old mice with osteoporotic bone, within two groups: OVX (ovariectomized) and sham (lumbar access, no ovariectomy group control). Laser beam was applied directly in a punch of skin in the distal medial part of tibia. To access bone quality, we calculate mineral/matrix ratio, relative lipid and proteoglycan content as well as the crystallinity using normalized spectrum within the integrated area method. The variables obtained for bone quality were statistically verify by ANOVA and tested for normality, where differences were considered to be significant for P < 0.05. In osteoporotic bone (OVX) we found a decreasing ratio between mineral to matrix and relative proteoglycan content, followed by a relative increasing lipid content when comparing with healthy bone (Sham) with statistical significance. The crystallinity showed higher value for OVX group but without statistical significance. Our Confocal Raman Spectroscopy provides a well-controlled environment to differentiate osteoporotic bone from healthy bone by decreasing calcium and glycosaminoglycans and increasing the amount of lipids in the cortical tibia of the mouse in vivo.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S092420311830122X

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