Maturação in vitro de oócitos humanos e posterior fertilização

Abstract

A prospective non randomized, descriptive study was carried out, during the period of November of 1999 until March of 2001, with 20 cycles of in vitro fertilization (IVF) of 15 patients with tubal infertility. The aim of this study was to retrieve immature oocytes for in vitro maturation, in vitro fertilization and embryo transfer in order to achieve pregnancy. The patients were recruited at the Human reproduction Center of Hospital das Clínicas and at out patients department of infertility from Posto de Atendimento Médico (PAM) Sagrada Família. All of them have signed the written consent before the studys beginning. The protocol was approved by board of Gynecology and Obstetrics. The selected patients were at least 18 and at most 32 years of age not completed and received 300UI of follicle stimulating hormone (FSH) recombinant by intramuscular injection at second day of the cycle and additional doses of 150UI at fourth and sixth day of cycle. The oocyte retrieval was performed always at seventh day of the cycle. Those oocytes classified as immatures were cultured in TCM-199 medium with antibiotics, pyruvate, FSH, human corionic gonadotropin (hCG) and serum (serum substitute supplement Irvine Scientific®). After 48 hours of culture oocytes that achieved metaphase II (MII) stage were inseminated, and the fertilized ones transfered. 144 follicles were aspirated, 67 immature oocytes were recovered, 43 achieved the MII stage and were inseminated. 30 fertilized and 25 embryos were transfered to 10 patients. There was one pregnancy with a baby born. We conclude that to mature in vitro human oocytes before in vitro fertilization is a procedure able to achieve pregnancy.