Intrauterine infection by mycobacterium avium subsp. paratuberculosis in buffalo (bubalus bubalis)

dc.creatorAlessandra Dos Santos Belo-Reis
dc.creatorJosé Diomedes Barbosa
dc.creatorFelipe Masiero Salvarani
dc.creatorM. F. Brito
dc.creatorAntônio Augusto Fonseca Junior
dc.creatorN. S. Silva
dc.creatorJalcides A. S. Silveira
dc.creatorJenner Karlisson Pimenta Dos Reis
dc.creatorJenevaldo Barbosa da Silva
dc.creatorCarlos M. C. Oliveira
dc.date.accessioned2025-05-28T14:20:56Z
dc.date.accessioned2025-09-09T01:17:30Z
dc.date.available2025-05-28T14:20:56Z
dc.date.issued2016
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.identifier.issn23259590
dc.identifier.urihttps://hdl.handle.net/1843/82554
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.rightsAcesso Aberto
dc.subjectMedicina Veterinária
dc.subject.otherBuffalo
dc.subject.otherTuberculosis in animals
dc.subject.otherEpidemiology
dc.titleIntrauterine infection by mycobacterium avium subsp. paratuberculosis in buffalo (bubalus bubalis)
dc.title.alternativeIntrauterine infection by mycobacterium avium subsp. paratuberculosis in buffalo (bubalus bubalis)
dc.typeArtigo de periódico
local.citation.epage3
local.citation.issue2
local.citation.spage1
local.citation.volume5
local.description.resumoThis study reports the detection of Mycobacterium avium subsp. paratuberculosis (Map) in the uterus and fetus of a buffalo (Bubalus bubalis). Samples of the buffalo cow’s ileum, mesenteric lymph node, uterus and placenta as well as various fetal organs were collected. Quantitative real-time polymerase chain reaction (qPCR), histopathology (hematoxylin-eosin (HE) staining) and Ziehl-Neelsen (ZN) staining were performed. Histopathological analysis revealed lesions consistent with paratuberculosis (PTB) in the mesenteric lymph node and small intestine, and ZN staining revealed the presence of acidalcohol resistant bacilli (AARB). Histological lesions were not identified in fetal tissues. qPCR indicated that uterus, mesenteric lymph node and small intestine samples from the buffalo cow were positive for Map. In contrast, in the fetus, only samples from the digestive tract were positive for Map based on qPCR data. These results indicate that Map is present in various buffalo organs and tissues, including within the reproductive system. Therefore, the intrauterine transmission of Map may occur in buffaloes and could represent an important source of infection.
local.publisher.countryBrasil
local.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA
local.publisher.initialsUFMG
local.url.externahttp://www.scitechnol.com/peer-review/intrauterine-infection-by-mycobacterium-avium-subsp-paratuberculosis-in-buffalo-bubalus-bubalis-JTnc.php?article_id=4591

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