Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis

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dc.creatorEderson Paulo Xavier Guilherme
dc.creatorJocilane Pereira de Oliveira
dc.creatorLorendane Millena de Carvalho
dc.creatorIgor Viana Brandi
dc.creatorSérgio Henrique Sousa Santos
dc.creatorGleidson Giordano Pinto de Carvalho
dc.creatorJúnio Cota Silva
dc.creatorBruna Mara Aparecida de Carvalho
dc.date.accessioned2022-08-25T14:05:05Z
dc.date.accessioned2025-09-09T00:23:02Z
dc.date.available2022-08-25T14:05:05Z
dc.date.issued2017-11
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.identifier.doihttps://doi.org/10.1002/elps.201700208
dc.identifier.issn1522-2683
dc.identifier.urihttps://hdl.handle.net/1843/44564
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofElectrophoresis
dc.rightsAcesso Restrito
dc.subjectAmilases
dc.subjectHidrólise
dc.subjectMaltose
dc.subjectBiorreatores
dc.titleSynthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis
dc.typeArtigo de periódico
local.citation.epage2946
local.citation.issue22-23
local.citation.spage2940
local.citation.volume38
local.description.resumoA bioreactor was built by means of immobilizing alpha-amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. The maximum value for starch hydrolysis was achieved at pH 5.0, concentration of immobilized enzyme 111.44 mg amylase/gcryogel, concentration of starch solution 45 g/L and temperature of 35°C. The immobilized enzyme showed a conversion ratio ranging from 68.2 to 97.37%, depending on the pH and temperature employed. Thus, our results suggest that the alpha-amylase from A. oryzae immobilized on cryogel monoliths represents a potential process for industrial production of maltose from starch hydrolysis.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.201700208

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