Use of the polymerase chain reaction as a complementary method for the detection of central nervous system involvement in children and adolescents with acute lymphoblastic leukemia

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dc.creatorCamila Silva Peres Cancela
dc.creatorJuliana Godoy Assumpção
dc.creatorFrancisco Danilo Ferreira de Paula
dc.creatorMitiko Murao
dc.creatorMarcos Borato Viana
dc.creatorBenigna Maria de Oliveira
dc.date.accessioned2023-07-19T19:52:31Z
dc.date.accessioned2025-09-08T23:15:39Z
dc.date.available2023-07-19T19:52:31Z
dc.date.issued2018
dc.identifier.doi10.7754/clin.lab.2017.170622
dc.identifier.issn14336510
dc.identifier.urihttps://hdl.handle.net/1843/56766
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofClinical Laboratory
dc.rightsAcesso Restrito
dc.subjectLeucemia-Linfoma Linfoblástico de Células Precursoras
dc.subjectVazamento de Líquido Cefalorraquidiano
dc.subjectLíquido Cefalorraquidiano
dc.subjectSistema Nervoso Central
dc.subjectReação em Cadeia da Polimerase
dc.subjectDiagnóstico
dc.subject.otheracute lymphoblastic leukemia
dc.subject.othercentral nervous system
dc.subject.otherCerebrospinal fluid
dc.subject.otherpolymerase chain reaction
dc.subject.otherdiagnosis
dc.titleUse of the polymerase chain reaction as a complementary method for the detection of central nervous system involvement in children and adolescents with acute lymphoblastic leukemia
dc.typeArtigo de periódico
local.citation.epage209
local.citation.spage205
local.citation.volume64
local.description.resumoBackground: Cytological analysis of the cerebrospinal fluid (CSF) remains the most widely used method for diagnosing central nervous system (CNS) involvement in acute lymphoblastic leukemia (ALL). This study aimed at evaluating the use of polymerase chain reaction (PCR), in comparison to other methods, for the assessment of the presence of blast cells in the CSF at the time of diagnosis of ALL. Methods: This was a prospective, single-centre, study enrolling all patients up to the age of 18 years who were admitted to a university hospital between November 2011 and November 2014 with a diagnosis of ALL and from whom it was possible to draw a sufficient amount of CSF for analysis by conventional cytology (CT), immunophenotyping (IMP), and PCR.Results: A total of 46 CSF samples from 44 ALL pediatric patients were included. CT was performed in all samples, IMP in 44, and PCR in 34. Thirteen (28.2%) samples showed positive results: two by CT, four by IMP, four by PCR, and three by both IMP and PCR.Conclusions: The results of this study showed that PCR should be considered a complementary method for the evaluation of the CSF in ALL patients at diagnosis.
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE PEDIATRIA
local.publisher.initialsUFMG
local.url.externahttps://www.clin-lab-publications.com/article/2622

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