Trametes villosa Lignin Peroxidase (TvLiP): genetic and molecular characterization

Página do item simplificado
dc.creatorRita Terezinha de Oliveira Carneiro
dc.creatorMaíza Alves Lopes
dc.creatorMarília Lôrdelo Cardoso Silva
dc.creatorVerônica da Silva Santos
dc.creatorVolnei Brito de Souza
dc.creatorAurizângela Oliveira de Sousa
dc.creatorCarlos Priminho Pirovani
dc.creatorMaria Gabriela Bello Koblitz
dc.creatorRaquel Guimarães Benevides
dc.creatorAristóteles Góes Neto
dc.date.accessioned2023-06-20T19:27:43Z
dc.date.accessioned2025-09-08T23:01:18Z
dc.date.available2023-06-20T19:27:43Z
dc.date.issued2017
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doihttp://dx.doi.org/10.4014/jmb.1606.06055
dc.identifier.issn1738-8872
dc.identifier.urihttps://hdl.handle.net/1843/55171
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of microbiology and biotechnology
dc.rightsAcesso Aberto
dc.subjectLignina
dc.subjectEnzimas
dc.subjectBasidiomycota - enzimologia
dc.subject.otherLignin
dc.subject.otherLigninolytic enzymes
dc.subject.otherWhite-rot basidiomycetes
dc.titleTrametes villosa Lignin Peroxidase (TvLiP): genetic and molecular characterization
dc.typeArtigo de periódico
local.citation.epage188
local.citation.issue1
local.citation.spage179
local.citation.volume27
local.description.resumoWhite-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the 7th day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.
local.identifier.orcidhttps://orcid.org/0000-0002-7568-6487
local.identifier.orcidhttps://orcid.org/0000-0002-5963-314X
local.identifier.orcidhttps://orcid.org/0000-0001-9669-7890
local.identifier.orcidhttps://orcid.org/0000-0002-6176-4069
local.identifier.orcidhttps://orcid.org/0000-0002-5558-570X
local.identifier.orcidhttps://orcid.org/0000-0002-5410-6973
local.identifier.orcidhttps://orcid.org/0000-0002-7692-6243
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1606.06055

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Trametes villosa Lignin Peroxidase (TvLiP)_ Genetic and Molecular Characterization.pdf
Tamanho:
2.32 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG