Enhanced efficacy against bacterial biofilms via host:guest cyclodextrin-doxycycline inclusion complexes

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dc.creatorPedro Pires Goulart Guimarães
dc.creatorAndressa Coelho de Menezes
dc.creatorKarina Imaculada Rosa Teixeira
dc.creatorÂngelo Márcio Leite Denadai
dc.creatorRichard Alfonso Fills Cerchar
dc.creatorMaría Esperanza Cortés Segura
dc.creatorRubén Dario Sinisterra Millán
dc.date.accessioned2023-11-24T14:22:28Z
dc.date.accessioned2025-09-09T00:56:40Z
dc.date.available2023-11-24T14:22:28Z
dc.date.issued2021-10
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)
dc.identifier.doihttps://doi.org/10.1007/s10847-020-01041-7
dc.identifier.issn1573-1111
dc.identifier.urihttps://hdl.handle.net/1843/61342
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Inclusion Phenomena and Macrocyclic Chemistry
dc.rightsAcesso Restrito
dc.subjectCiclodextrinas
dc.subjectSistemas de distribuição de medicamentos
dc.subjectBiofilme
dc.subjectDoença periodontal
dc.subjectPeriodontite
dc.subjectAgentes antibacterianos
dc.subjectLuz - Espalhamento
dc.subjectPotencial zeta
dc.subjectCalorimetria
dc.subject.otherDoxycycline
dc.subject.otherHydroxypropyl-β-cyclodextrin
dc.subject.otherInclusion complexes
dc.subject.otherDrug delivery
dc.subject.otherAggregatibacter actinomycetemcomitans
dc.subject.otherBiofilm
dc.titleEnhanced efficacy against bacterial biofilms via host:guest cyclodextrin-doxycycline inclusion complexes
dc.typeArtigo de periódico
local.citation.epage207
local.citation.issue3-4
local.citation.spage197
local.citation.volume99
local.description.resumoPeriodontal disease is characterized by a microbial infection and it is one of the major causes of teeth loss. The growth of pathogenic bacteria in oral cavity, such as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), provides a favorable enviromment for the biofilm formation, which result in periodontal diseases. The development of new technologies to potentiate existing drugs, avoiding bacterial biofilms resistance and improving chemical stability is highly desirable. Here, we report the use of host–guest chemistry to enhance the activity of antibacterial doxycycline (DOX) in A. actinomycetemcomitans bacterial strain suspension and biofilm in vitro through complexation with hydroxypropyl-β-cyclodextrin (HPβCD) using different molar ratios of DOX/HPβCD. 2D ¹H NMR confirmed the host–guest complexation of DOX and HPβCD. We assessed the colloidal characteristics of the complex DOX/HPβCD via Dynamic Light Scattering (DLS) and Zeta Potential (PZ). The mixing ratio 1:2 DOX/HPβCD significantly decreased the minimum inhibitory concentration (MIC) and improved efficacy against A. actinomycetemcomitans suspensions and biofilms, respectively, when compared to free DOX and other DOX/HPβCD complexes. Further, the interaction of different molar ratio proportions of DOX/HPβCD complex with bacterial membrane was demonstrated via Isothermal Titration Calorimetry (ITC). Thus, we suggested the enhanced efficacy of the DOX/HPβCD complexes, at molar ratio 1:2, is due the higher cyclodextrin ratio, which potentiate the interaction between drug and bacterial membrane through nonionic interactions, such as hydrogen bonding or other van der Waals interactions. Collectively, the development of these complexes enables increased efficacy against bacterial biofilms, which hold promise for the treatment of aggressive and non-responsive forms of periodontitis.
local.identifier.orcidhttps://orcid.org/0000-0003-4534-2779
local.identifier.orcidhttps://orcid.org/0000-0001-8259-6933
local.identifier.orcidhttps://orcid.org/0000-0002-1298-7581
local.identifier.orcidhttps://orcid.org/0000-0002-0560-8491
local.identifier.orcidhttps://orcid.org/0000-0001-7656-1849
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s10847-020-01041-7

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