Neutrophil extracellular traps in rheumatoid arthritis and periodontitis: contribution of PADI4 gene polymorphisms

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dc.creatorSicília Rezende Oliveira
dc.creatorSandra Yasuyo Fukada Alves
dc.creatorPaula Rocha Moreira
dc.creatorRenê Donizeti Ribeiro de Oliveira
dc.creatorPaulo Louzada-Junior
dc.creatorLucas Guimarães Abreu
dc.creatorFernando de Queiroz Cunha
dc.creatorTarcília Aparecida da Silva
dc.creatorJosé Alcides Almeida de Arruda
dc.creatorAyda Henriques Schneider
dc.creatorLarissa Marques Bemquerer
dc.creatorRayssa Maria Soalheiro de Souza
dc.creatorPaula Barbim Donate
dc.creatorGustavo Henrique de Mattos-Pereira
dc.creatorDébora Cerqueira Calderaro
dc.creatorCaio Cavalcante Machado
dc.date.accessioned2025-05-16T19:20:16Z
dc.date.accessioned2025-09-09T00:28:46Z
dc.date.available2025-05-16T19:20:16Z
dc.date.issued2024-04
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jcpe.13921
dc.identifier.issn1600-051X
dc.identifier.urihttps://hdl.handle.net/1843/82332
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of clinical periodontology
dc.rightsAcesso Restrito
dc.subjectExtracellular traps
dc.subjectPeriodontitis
dc.subjectProtein-arginine deiminase type 4
dc.subjectArthritis, rheumatoid
dc.subjectPolymorphism, single nucleotide
dc.subjectHaplotypes
dc.subjectPeroxidase
dc.subjectBiomarkers
dc.subject.otherNeutrophil extracellular traps
dc.subject.otherPeriodontitis
dc.subject.otherProtein-arginine deiminase type 4
dc.subject.otherRheumatoid arthritis
dc.subject.otherSingle nucleotide polymorphism
dc.titleNeutrophil extracellular traps in rheumatoid arthritis and periodontitis: contribution of PADI4 gene polymorphisms
dc.typeArtigo de periódico
local.citation.epage463
local.citation.issue4
local.citation.spage452
local.citation.volume51
local.description.resumoAim: We sought to investigate the release of neutrophil extracellular traps (NETs) in neutrophils from individuals with rheumatoid arthritis (RA) and controls and compare the presence of NETs in gingival tissues according to periodontal status. Also, the association between single nucleotide polymorphisms (SNPs) of the peptidyl arginine deaminase type 4 (PADI4) gene and the GTG haplotype with RA, periodontitis and NETs was evaluated in vitro. Materials and methods: Peripheral neutrophils were isolated by density gradient, and NET concentration was determined by the PicoGreen method. Immunofluorescence was studied to identify NETs by co-localization of myeloperoxidase (MPO)-citrullinated histone H3 (H3Cit). Genotyping for SNPs (PADI4_89; PADI4_90; PADI4_92; and PADI4_104) was performed in 87 individuals with RA and 111 controls. Results: The release of NETs in vitro was significantly higher in individuals with RA and periodontitis and when stimulated with Porphyromonas gingivalis. Gingival tissues from subjects with RA and periodontitis revealed increased numbers of MPO-H3Cit-positive cells. Individuals with the GTG haplotype showed a higher release of NETs in vitro and worse periodontal parameters. Conclusions: The release of NETs by circulating neutrophils is associated with RA and periodontitis and is influenced by the presence of the GTG haplotype.
local.identifier.orcidhttps://orcid.org/0000-0002-8164-2122
local.identifier.orcidhttps://orcid.org/0000-0002-6599-3950
local.identifier.orcidhttps://orcid.org/0000-0003-2474-2773
local.identifier.orcidhttps://orcid.org/0000-0003-2037-9532
local.identifier.orcidhttps://orcid.org/0000-0003-0176-0741
local.identifier.orcidhttps://orcid.org/0000-0001-5535-3223
local.identifier.orcidhttps://orcid.org/0000-0002-6589-4784
local.identifier.orcidhttps://orcid.org/0000-0003-2585-3870
local.identifier.orcidhttps://orcid.org/0000-0003-2258-8071
local.identifier.orcidhttps://orcid.org/0000-0003-4755-1670
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0009-0006-1090-2165
local.identifier.orcidhttps://orcid.org/0000-0001-8422-8894
local.identifier.orcidhttps://orcid.org/0000-0003-1078-6281
local.identifier.orcidhttps://orcid.org/0000-0003-0229-6864
local.identifier.orcidhttps://orcid.org/0000-0003-1619-1959
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOPEDIATRIA E ORTODONTIA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE APARELHO LOCOMOTOR
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jcpe.13921

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