Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice

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dc.creatorCamila Henriques Coelho
dc.creatorAdriana Oliveira Costa
dc.creatorAna Carolina Carvalho Silva
dc.creatorMaíra Mazzoni Pucci
dc.creatorÂngela Vieira Serufo
dc.creatorHaendel Gonçalves Nogueira Oliveira Busatti
dc.creatorMaurício Durigan
dc.creatorJonas Enrique Aguilar Perales
dc.creatorDonat Alexander de Chapeaurouge
dc.creatorDaniel Almeida da Silva e Silva
dc.creatorMaria Aparecida Gomes
dc.creatorJuliano Simões Toledo
dc.creatorSteven M. Singer
dc.creatorRosiane Aparecida da Silva Pereira
dc.creatorAna Paula Salles Moura Fernandes
dc.date.accessioned2022-03-15T17:53:25Z
dc.date.accessioned2025-09-09T00:27:05Z
dc.date.available2022-03-15T17:53:25Z
dc.date.issued2016
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0164946
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1843/40109
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPlos One
dc.rightsAcesso Aberto
dc.subjectGiardíase
dc.subjectGiardia lamblia
dc.subjectProteômica
dc.subjectTrofozoítos
dc.subjectFilogenia
dc.titleGenotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to mice
dc.typeArtigo de periódico
local.citation.epage15
local.citation.issue10
local.citation.spage1
local.citation.volume11
local.description.resumoThe zoonotic potential of giardiasis, as proposed by WHO since the late 70's, has been largely confirmed in this century. The genetic assemblages A and B of Giardia duodenalis are frequently isolated from human and canine hosts. Most of the assemblage A strains are not infective to adult mice, which can limit the range of studies regarding to biology of G. duodenalis, including virulence factors and the interaction with host immune system. This study aimed to determine the infectivity in mice of an assemblage A Giardia duodenalis strain (BHFC1) isolated from a dog and to classify the strain in sub-assemblages (AI, AII, AIII) through the phylogenetic analysis of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. In addition, the proteomic profile of soluble and insoluble protein fractions of trophozoites was analyzed by 2D-electrophoresis. Accordingly, trophozoites of BHFC1 were highly infective to Swiss mice. The phylogenetic analysis of tpi and gdh revealed that BHFC1 clustered to sub-assemblage AI. The proteomic map of soluble and insoluble protein fractions led to the identification of 187 proteins of G. duodenalis, 27 of them corresponding to hypothetical proteins. Considering both soluble and soluble fractions, the vast majority of the identified proteins (n = 82) were classified as metabolic proteins, mainly associated with carbon and lipid metabolism, including 53 proteins with catalytic activity. Some of the identified proteins correspond to antigens while others can be correlated with virulence. Besides a significant complementation to the proteomic data of G. duodenalis, these data provide an important source of information for future studies on various aspects of the biology of this parasite, such as virulence factors and host and pathogen interactions.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164946

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