Paclitaxel-loaded pH-sensitive liposome: new insights on structural and physicochemical characterization

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dc.creatorLiziane Oliveira Fonseca Monteiro
dc.creatorÂngelo Malachias de Souza
dc.creatorGwenaelle Elza Nathalie Pound-Lana
dc.creatorRogério Magalhães Paniago
dc.creatorVanessa Carla Furtado Mosqueira
dc.creatorMônica Cristina de Oliveira
dc.creatorAndré Luís Branco de Barros
dc.creatorElaine Amaral Leite
dc.date.accessioned2023-03-24T19:47:41Z
dc.date.accessioned2025-09-08T23:52:54Z
dc.date.available2023-03-24T19:47:41Z
dc.date.issued2018
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1021/acs.langmuir.8b00411
dc.identifier.issn1520-5827
dc.identifier.urihttps://hdl.handle.net/1843/51211
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofLangmuir
dc.rightsAcesso Restrito
dc.subjectTransições de fases
dc.subjectLipossomos
dc.subjectTumores
dc.subject.otherLattices
dc.subject.otherPhase transitions
dc.subject.otherVesicles
dc.subject.otherX-ray scattering
dc.subject.otherpH
dc.titlePaclitaxel-loaded pH-sensitive liposome: new insights on structural and physicochemical characterization
dc.typeArtigo de periódico
local.citation.epage5737
local.citation.issue20
local.citation.spage5728
local.citation.volume34
local.description.resumoA long-circulating and pH-sensitive liposome containing paclitaxel (SpHL-PTX) was recently developed by our group. Once in an acidic environment, for example, tumors, these liposomes undergo destabilization, releasing the encapsulated drug. In this way, the aim of this study was to evaluate the molecular and supramolecular interactions between the lipid bilayer and PTX in similar biological environment conditions. High-sensitivity analyses of SpHL-PTX structures were obtained by the small-angle X-ray scattering technique combined with other techniques such as dynamic light scattering, asymmetric flow field-flow fractionation, transmission electron microscopy, and high-performance liquid chromatography. The results showed that PTX incorporation in the liposomal bilayer clearly leads to changes in supramolecular organization of dioleoylphosphatidylethanolamine (DOPE) molecules, inducing the formation of more ordered structures. Changes in supramolecular organization were observed at lower pH, indicating that pH sensitivity was preserved even in the presence of fetal bovine serum proteins. Furthermore, morphological and physicochemical characterization of SpHL-PTX evidenced the formation of nanosized dispersion suitable for intravenous administration. In conclusion, a stable nanosized dispersion of PTX was obtained at pH 7.4 with suitable parameters for intravenous administration. At lower pH conditions, the pH sensitivity of the system was clearly evidenced by changes in the supramolecular organization of DOPE molecules, which is crucial for the delivery of PTX into the cytoplasm of the targeted cells. In this way, the results obtained by different techniques confirm the feasibility of SpHL as a promising tool to PTX delivery in acidic environments, such as tumors.
local.identifier.orcidhttps://orcid.org/0000-0002-8703-4283
local.identifier.orcidhttps://orcid.org/0000-0003-0569-0242
local.identifier.orcidhttps://orcid.org/0000-0002-5203-0944
local.identifier.orcidhttps://orcid.org/0000-0002-0368-6090
local.identifier.orcidhttps://orcid.org/0000-0002-7641-6585
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.departmentICX - DEPARTAMENTO DE FÍSICA
local.publisher.initialsUFMG
local.url.externahttps://pubs.acs.org/doi/10.1021/acs.langmuir.8b00411

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