Droplet digital PCR for Acinetobacter baumannii diagnosis in bronchoalveolar lavage samples from patients with ventilator-associated pneumonia

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dc.creatorMirna Giselle Moreira
dc.creatorAnna Gabriella Guimarães Oliveira
dc.creatorIhtisham Ul Haq
dc.creatorTatiana Flávia Pinheiro de Oliveira
dc.creatorWadi Alonazi
dc.creatorAntônio Augusto Fonseca Júnior
dc.creatorVandack Alencar Nobre Junior
dc.creatorSimone Gonçalves dos Santos
dc.date.accessioned2026-01-14T20:55:58Z
dc.date.issued2024-09-13
dc.identifier.doihttps://doi.org/10.3390/antibiotics13090878
dc.identifier.issn2079-6382
dc.identifier.urihttps://hdl.handle.net/1843/1390
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofAntibiotics
dc.rightsAcesso aberto
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectReação em Cadeia da Polimerase em Tempo Real
dc.subjectPneumonia Associada à Ventilação Mecânica
dc.subjectDiagnóstico
dc.subject.otherAcinetobacter baumannii
dc.subject.otherDroplet digital PCR
dc.subject.otherReal-time PCR
dc.subject.otherDiagnosis
dc.subject.otherVentilator-associated pneumonia
dc.titleDroplet digital PCR for Acinetobacter baumannii diagnosis in bronchoalveolar lavage samples from patients with ventilator-associated pneumonia
dc.typeArtigo de periódico
local.citation.epage14
local.citation.issue9
local.citation.spage1
local.citation.volume13
local.description.resumoAdvanced diagnostic technologies have made accurate and precise diagnosis of pathogens easy. Herein, we present a new diagnostic method, droplet digital PCR (ddPCR), to detect and quantify Acinetobacter baumannii in mini bronchoalveolar lavage (mini-BAL) samples. A. baumannii causes ventilator-associated pneumonia (VAP), a severe healthcare infection affecting patients’ lungs. VAP carries a high risk of morbidity and mortality, making its timely diagnosis crucial for prompt and effective management. Methodology. The assay performance was evaluated by comparing colonization data, quantitative culture results, and different generations of PCR (traditional PCR and Real-Time PCR—qPCR Taqman® and SYBR® Green). The ddPCR and qPCR Taqman® prove to be more sensitive than other molecular techniques. Reasonable analytical specificity was obtained with ddPCR, qPCR TaqMan®, and conventional PCR. However, qPCR SYBR® Green technology presented a low specificity, making the results questionable in clinical samples. DdPCR detected/quantified A. baumanni in more clinical samples than other methods (38.64% of the total samples). This emerging ddPCR technology offers promising advantages such as detection by more patients and direct quantification of pathogens without calibration curves.
local.publisher.countryBrasil
local.publisher.departmentMEDICINA - FACULDADE DE MEDICINA
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS DA SAUDE::MEDICINA
local.url.externahttps://www.mdpi.com/journal/antibiotics

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