Caracterização genotípica de Staphylococcus aureus resistentes à meticilina isolados de tetos e leite bovino

Abstract

The present study aimed to verify the presence of the mecA and mecA LGA251 genes and blaZ in isolates of S. aureus previously identified and resistant to beta-lactams antibiotics, from samples of cows diagnosed with subcli-nical mastitis on the surface of bovine ceilings herds in north of Minas Gerais. Isolates previously identified through the detection of the femA gene and MALDI-TOF MS and resistant to antibiotics of the beta-lactams group were used. The isolates were subjected to DNA extraction and the screening of resistance genes was performed using the PCR technique. Although phenotypically resistant to oxacillin and cefoxitin, none of the strains showed positive results for the mecA and mecALGA251 genes in the tests performed, their absence does not rule out the possibility of the presence of other resistance genes in the rural properties under study. However, the presence of the blaZ gene was verified in 4 (12.90%) of the isolates under study. The results highlight the need for monitoring and adoption of control measures that prevent the spread of these genes that represent a great risk to public health.