Characterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions

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dc.creatorJéssica Amanda Marques Souza
dc.creatorFrédéric Jean Georges Frézard
dc.creatorDaniele da Glória de Souza
dc.creatorMauro Martins Teixeira
dc.creatorFrederico Marianetti Soriani
dc.creatorLudmila de Matos Baltazar
dc.creatorVirgínia Mendes Carregal
dc.creatorLudmila Gouveia Eufrasio
dc.creatorAndré Gustavo de Oliveira
dc.creatorWendell Girard Dias
dc.creatorKildare Rocha de Miranda
dc.creatorIran Malavazi
dc.creatorDaniel de Assis Santos
dc.date.accessioned2023-07-14T22:45:34Z
dc.date.accessioned2025-09-09T00:14:58Z
dc.date.available2023-07-14T22:45:34Z
dc.date.issued2019
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.02008
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/1843/56331
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in Microbiology
dc.rightsAcesso Aberto
dc.subjectAspergillus fumigatus
dc.subjectMacrófagos
dc.subjectNeutrofilos
dc.subject.otherExtracellular vesicles
dc.subject.otherFlamentous fungus
dc.subject.otherAspergillus fumigatus
dc.subject.otherHost-pathogen interactions
dc.subject.otherMacrophages
dc.subject.otherNeutrophils
dc.titleCharacterization of aspergillus fumigatus axtracellular vesicles and their effects on macrophages and neutrophils functions
dc.typeArtigo de periódico
local.citation.epage13
local.citation.spage1
local.citation.volume10
local.description.resumoExtracellular vesicles (EVs) has been considered an alternative process for intercellular communication. EVs release by filamentous fungi and the role of vesicular secretion during fungus-host cells interaction remain unknown. Here, we identified the secretion of EVs from the pathogenic filamentous fungus, Aspergillus fumigatus. Analysis of the structure of EVs demonstrated that A. fumigatus produces round shaped bilayer structures ranging from 100 to 200 nm size, containing ergosterol and a myriad of proteins involved in REDOX, cell wall remodeling and metabolic functions of the fungus. We demonstrated that macrophages can phagocytose A. fumigatus EVs. Phagocytic cells, stimulated with EVs, increased fungal clearance after A. fumigatus conidia challenge. EVs were also able to induce the production of TNF-α and CCL2 by macrophages and a synergistic effect was observed in the production of these mediators when the cells were challenged with the conidia. In bone marrow-derived neutrophils (BMDN) treated with EVs, there was enhancement of the production of TNF-α and IL-1β in response to conidia. Together, our results demonstrate, for the first time, that A. fumigatus produces EVs containing a diverse set of proteins involved in fungal physiology and virulence. Moreover, EVs are biologically active and stimulate production of inflammatory mediators and fungal clearance.
local.identifier.orcidhttps://orcid.org/0000-0003-3783-5717
local.identifier.orcidhttps://orcid.org/0000-0002-6944-3008
local.identifier.orcidhttps://orcid.org/0000-0003-4720-6746
local.identifier.orcidhttps://orcid.org/0000-0002-4255-3047
local.identifier.orcidhttps://orcid.org/0000-0001-9152-8756
local.identifier.orcidhttps://orcid.org/0000-0001-8186-8714
local.identifier.orcidhttps://orcid.org/0000-0001-9799-773X
local.identifier.orcidhttps://orcid.org/0000-0002-1108-5666
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02008/full

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