Nestin and NG2 transgenes reveal two populations of perivascular cells stimulated by photobiomodulation

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dc.creatorNatália Amanda Gomes
dc.creatorPedro Henrique Dias Moura Prazeres
dc.creatorGustavo Batista de Menezes
dc.creatorCláudia Silami de Magalhães
dc.creatorRicardo Alves de Mesquita
dc.creatorMárcia Martins Marques
dc.creatorAlexander Birbrair
dc.creatorIvana Márcia Alves Diniz
dc.creatorIsabella B. Valle
dc.creatorFrederico Omar Gleber-Netto
dc.creatorTarcília Aparecida da Silva
dc.creatorHortência Maciel de Castro Oliveira
dc.creatorRafaela Ferrer de Oliveira
dc.creatorLuiza de Almeida Queiroz Ferreira
dc.creatorLia Silva de Castilho
dc.creatorPaulo H.R.G. Reis
dc.date.accessioned2025-05-07T19:49:40Z
dc.date.accessioned2025-09-08T23:24:41Z
dc.date.available2025-05-07T19:49:40Z
dc.date.issued2022-04
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1002/jcp.30680
dc.identifier.issn1097-4652
dc.identifier.urihttps://hdl.handle.net/1843/82119
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Cellular Physiology
dc.rightsAcesso Restrito
dc.subjectPericytes
dc.subjectLow-level light therapy
dc.subjectDental pulp
dc.subjectMinerals
dc.subjectVascular endothelial growth factor A
dc.subject.otherNeural progenitors
dc.subject.otherPericytes
dc.subject.otherPhotobiomodulation
dc.subject.otherPulp biology
dc.titleNestin and NG2 transgenes reveal two populations of perivascular cells stimulated by photobiomodulation
dc.typeArtigo de periódico
local.citation.epage2210
local.citation.issue4
local.citation.spage2198
local.citation.volume237
local.description.resumoPericytes and glial cells are known to collaborate in dental pulp tissue repair. Cell-based therapies that stimulate these stromal components may be of therapeutic relevance for partially vital dental pulp conditions. This study aimed to examine the early effect of photobiomodulation (PBM) in pericytes from experimentally injured pulp tissue. To accomplish this, we used the Nestin-GFP/NG2-DsRed mice, which could allow the identification of distinct pericyte phenotypes. We discovered the presence of two pericytes subsets within the dental pulp, the Nestin + NG2+ (type-2) and Nestin- NG2+ (type-1). Upon injury, PBM treatment led to a significant increase in Nestin+ cells and pericytes. This boost was mainly conferred by the more committed pericyte subset (NestinNG2+ ). PBM also stimulated terminal blood vessels sprouting adjacent to the injury site while maintaining signs of pulp vitality. In vitro, PBM induced VEGF upregulation, improved dental pulp cells proliferation and migration, and favored their mineralization potential. Herein, different subsets of perivascular cells were unveiled in the pulp tissue. PBM enhanced not only NG2+ cells but nestin-expressing progenitors in the injured dental pulp.
local.identifier.orcidhttps://orcid.org/0000-0002-5101-8089
local.identifier.orcidhttps://orcid.org/0000-0003-3207-4007
local.identifier.orcidhttps://orcid.org/0000-0002-9398-1252
local.identifier.orcidhttps://orcid.org/0000-0003-1015-2561
local.identifier.orcidhttps://orcid.org/0000-0003-4261-0037
local.identifier.orcidhttps://orcid.org/0000-0001-7847-9902
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0000-0002-4926-0618
local.identifier.orcidhttps://orcid.org/0000-0001-6572-5895
local.identifier.orcidhttps://orcid.org/0000-0002-5534-9432
local.identifier.orcidhttps://orcid.org/0000-0001-9648-6815
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1002/jcp.30680

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