Identification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil.

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dc.creatorJosiane dos Santos
dc.creatorAlessandra Rejane Ericsson de Oliveira Xavier
dc.creatorMauro Aparecido de Sousa Xavier
dc.creatorLéia Cardoso
dc.creatorSérgio Avelino Mota Nobre
dc.creatorRicardo Rodrigues Bacchi
dc.creatorCarlos Humbertp Chamone Cangussu
dc.creatorAnna Christina de Almeida
dc.creatorLuciana Nobre Leite
dc.creatorNair Amélia Prates Barreto
dc.date.accessioned2022-06-03T15:57:27Z
dc.date.accessioned2025-09-09T01:10:55Z
dc.date.available2022-06-03T15:57:27Z
dc.date.issued2020
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.identifier.doihttps://doi.org/10.4238/gmr18521
dc.identifier.issn1676-5680
dc.identifier.urihttps://hdl.handle.net/1843/42229
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofGenetics and Molecular Research
dc.rightsAcesso Aberto
dc.subjectLevedos
dc.subjectFilogenia
dc.titleIdentification and molecular analysis of yeast in droppings of domestic pigeons in Montes Claros, Brazil.
dc.typeArtigo de periódico
local.citation.issue1
local.citation.volume19
local.description.resumoPigeon droppings carry microorganisms such as Candida spp., which have high genetic variability and are the most common opportunistic pathogens in humans. There is a need to establish fast, reliable and simple laboratory methods to differentiate pathogenic from non-pathogenic yeast species in pigeon droppings, as they can be a source of contamination for humans. We examined yeasts isolated from domestic pigeon droppings through a proteomic approach using matrix-assisted laser desorption ionization (MALDI-TOF). We also examined genetic characteristics of the isolates by restriction fragment length polymorphism (RFLP) PCR and enterobacterial repetitive intergenic consensus sequences PCR methods. Five colonies isolated from domestic pigeon droppings collected at three different sampling locations on the roof of a Brazilian university building were identified as Candida krusei and Candida rugosa by MALDI-TOF. Phylogenetic studies using the two PCR-based molecular methods were able to differentiate the species C. krusei and C. rugosa into different groups. These genomic methods gave similar results and proved useful for genetic diversity analysis and interspecific differentiation of Candida isolates.
local.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.geneticsmr.com/articles/identification-and-molecular-analysis-yeasts-found-domestic-pigeon-droppings-montes-claros

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