Serologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil

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dc.creatorLuciana Fachini Costa
dc.creatorMarcos Xavier Silva
dc.creatorTatiane Alves Paixão
dc.creatorRenato Lima Santos
dc.creatorAnna Christina de Almeida
dc.creatorMoises Sena Pessoa
dc.creatorLais Bitencourt Guimarães
dc.creatorAnne Karoene Silva Faria
dc.creatorRodrigo Pereira Morão
dc.creatorJuliana Pinto da Silva Mol
dc.creatorLuize Néli Nunes Garcia
dc.creatorAnna Christina de Almeida
dc.creatorAurora Maria Guimarães Gouveia
dc.date.accessioned2022-06-01T14:32:58Z
dc.date.accessioned2025-09-08T23:44:08Z
dc.date.available2022-06-01T14:32:58Z
dc.date.issued2016
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doiDOI 10.1186/s13104-016-1998-2
dc.identifier.issn1756-0500
dc.identifier.urihttps://hdl.handle.net/1843/42163
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBMC Research Notes
dc.rightsAcesso Aberto
dc.subjectColibacilose
dc.subjectBrucelose
dc.subjectOvino
dc.subjectCaprino
dc.titleSerologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazil
dc.typeArtigo de periódico
local.citation.issue190
local.citation.volume9
local.description.resumoBackground: Brucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. This study aimed to determine the frequency of B. ovis positivity in rams and goats flocks in the State of Minas Gerais, Brazil, by agarose gel immunodiffusion (AGID), ELISA, Rose Bengal, PCR and bacteriological isolation as diagnostic tools. Findings: Serum and urine samples were collected from properties with sheep or goat flocks, or from properties with mixed flock. Out of 50 sheep flocks, 6 % (3/50) were seropositive by AGID while 4 % (2/50) were positive by urine PCR for B. ovis. Out of five goat farms, 20 % (1/5) were seropositive for B. ovis by AGID. Mixed flock farms had 11.1 % (2/18) of positivity by AGID. By ELISA, 19.5 % (8/41) of sheep properties and 61.1 % (11/18) of the properties with mixed flocks were positive for B. ovis. No samples were positive in the test of Rose Bengal, ruling out exposure to smooth LPS Brucella species (particularly Brucella melitensis) and indicating that the positive in the ELISA was associated with Brucella spp. LPS rough (presumably B. ovis). No urine sample from sheep or goat was positive by bacteriological isolation. Conclusions: Our results demonstrate serologic or molecular evidence of B. ovis infection in several rams and billy goats from meso-regions of the State of Minas Gerais, Brazil. Also, this study report the indirect ELISA as an important tool for the diagnosis of B. ovis infection, as indirect ELISA in this study demonstrated to be the most sensitive diagnostic method adopted.
local.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG

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