AAV-Mediated expression of miR-17 enhances neurite and axon regeneration in vitro

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dc.creatorRaquel Alves Almeida
dc.creatorCarolina Gomes Ferreira
dc.creatorVictor Ulysses Souza Matos
dc.creatorJulia Meireles Nogueira
dc.creatorMarina Pimenta Braga
dc.creatorLucas Caldi Gomes
dc.creatorErika Cristina Jorge
dc.creatorFrederico Marianetti Soriani
dc.creatorUwe Michel
dc.creatorVinicius Toledo Ribas
dc.date.accessioned2026-01-06T21:08:11Z
dc.date.issued2024-08-21
dc.identifier.doihttps://doi.org/10.3390/ijms25169057
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/1843/1298
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofInternational journal of molecular sciences
dc.rightsAcesso aberto
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.subjectGenetica
dc.subject.otherAxon
dc.subject.otherDamage
dc.subject.otherRegeneration
dc.subject.othermiR-17
dc.titleAAV-Mediated expression of miR-17 enhances neurite and axon regeneration in vitro
dc.typeArtigo de periódico
local.citation.epage16
local.citation.issue16
local.citation.spage1
local.citation.volume25
local.description.resumoNeurodegenerative disorders, including traumatic injuries to the central nervous system (CNS) and neurodegenerative diseases, are characterized by early axonal damage, which does not regenerate in the adult mammalian CNS, leading to permanent neurological deficits. One of the primary causes of the loss of regenerative ability is thought to be a developmental decline in neurons’ intrinsic capability for axon growth. Different molecules are involved in the developmental loss of the ability for axon regeneration, including many transcription factors. However, the function of microRNAs (miRNAs), which are also modulators of gene expression, in axon re-growth is still unclear. Among the various miRNAs recently identified with roles in the CNS, miR-17, which is highly expressed during early development, emerges as a promising target to promote axon regeneration. Here, we used adeno-associated viral (AAV) vectors to overexpress miR-17 (AAV.miR-17) in primary cortical neurons and evaluate its effects on neurite and axon regeneration in vitro. Although AAV.miR-17 had no significant effect on neurite outgrowth and arborization, it significantly enhances neurite regeneration after scratch lesion and axon regeneration after axotomy of neurons cultured in microfluidic chambers. Target prediction and functional annotation analyses suggest that miR-17 regulates gene expression associated with autophagy and cell metabolism. Our findings suggest that miR-17 promotes regenerative response and thus could mitigate neurodegenerative effects.
local.publisher.countryBrasil
local.publisher.departmentICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS BIOLOGICAS::GENETICA
local.url.externahttps://www.mdpi.com/1422-0067/25/16/9057

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