Circovirus suíno-2 em suídeos brasileiros: detecção viral pela imunoistoquímica e estudos sorológicos

Abstract

Standardized the technique of immunohistochemistry (IHC) using a commercial polyclonal antibody anti-PCV2 porcine origin to identify the Circovirus type 2 (PCV2). The technique was applied in tissues of pigs from farms with and without suspicion of Postweaning Multisystemic Wasting Syndrome (PMWS). The viral antigen was demonstrated in 74 (36.45%) of 203 samples analyzed, and lymph node and lung tissues where the virus was identified more frequently. Tembe was standardized technique imunoperozidase in monolayer cells (IPMC) aiming at the demonstration of antibodies to PCV2 pigs. Sera were diluted and incubated on the monolayer of PK15 cells previously infected with the virus and fixed. The technique allowed the differentiation and titration of sera, reagents and animals and establishment of non-reactive serological profile in eight commercial farms producing pigs with and without suspicion of SRM. In analyzing the results it was found that PCV2 infection occurred in all farms. In order to make a preliminary epidemiological study were also analyzed 955 sera from 35 commercial farms for pigs and wild boars from 1322 sera from 119 farms located in six states. The results showed that 96.49% and 87% responded to the presence of PCV2 in sera of pigs and boars, respectively, while the virus spread in Brazilian herds affected by the syndrome or not