Liposomes containing gadodiamide: preparation, physicochemical characterization, and in vitro cytotoxic evaluation

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dc.creatorAna Luiza Chaves Maia
dc.creatorChristian Fernandes
dc.creatorCynthia Nara Pereira de Oliveira
dc.creatorClaudia Salviano Teixeira
dc.creatorMariana Silva Oliveira
dc.creatorDaniel Crístian Ferreira Soares
dc.creatorGilson Andrade Ramaldes
dc.date.accessioned2022-05-30T20:38:20Z
dc.date.accessioned2025-09-09T00:28:08Z
dc.date.available2022-05-30T20:38:20Z
dc.date.issued2017
dc.identifier.doi10.2174/1567201813666160907095404
dc.identifier.issn1567-2018
dc.identifier.urihttps://hdl.handle.net/1843/42086
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCurrent Drug Delivery
dc.rightsAcesso Restrito
dc.subjectAtividade citotóxica
dc.subjectLipossomas
dc.subjectGadodiamida
dc.subjectCaracterização físico-química
dc.subject.otherAntineoplastic agent
dc.subject.otherLiposomes
dc.subject.otherCancer treatment
dc.subject.other4T1 breast cancer cell line
dc.subject.otherGd-DTPA-BMA
dc.subject.otherIn vitro cytotoxic evaluation
dc.titleLiposomes containing gadodiamide: preparation, physicochemical characterization, and in vitro cytotoxic evaluation
dc.typeArtigo de periódico
local.citation.epage574
local.citation.issue4
local.citation.spage566
local.citation.volume14
local.description.resumoBackground: The aim of this study was to develop, characterize and assess the cytotoxic activity of pHsensitive (pHL-Gd), stealth pH-sensitive (SpHL-Gd), and conventional (convL-Gd) liposomes containing gadodiamide (Gd-DTPA-BMA). Methods: Formulations were prepared by reverse-phase evaporation method and their physicochemical properties were evaluated by means of particle size, zeta potential, and Gd-DTPA-BMA entrapment. SpHL-Gd was considered being the most promising liposome, since it combines stealth and fusogenic characteristics that might contribute to achieve higher therapeutic efficiency. Their drug encapsulation percentages have been optimized satisfactorily. The addition of Gd-DTPA-BMA at 125 μmol/mL in the SpHL-Gd preparation allowed obtaining liposomes with appropriate encapsulation percentage (20.3 ± 0.1%) and entrapment (25.4 ± 0.1 μmol/mL). Results: The cytotoxic studies on the 4T1 breast cancer cell line demonstrated that liposomes-loaded with Gd-DTPA-BMA inhibited cancer cell. pHL-Gd and SpHL-Gd liposomes showed higher activity than convL-Gd and free Gd-DTPA-BMA, indicating that the pH-sensitive characteristic was important to improve intracellular delivery. Conclusion: The presence of polyethylene glycol (PEG) in the SpHL-Gd formulation did not affect the pH-sensitivity and internalization. Therefore, the results of this study suggest the feasibility of liposomes containing Gd-DTPA-BMA as a new promising controlled delivery system.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.initialsUFMG
local.url.externahttps://www.eurekaselect.com/article/78251

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