Extracellular vesicles from adipose-derived mesenchymal stem/stromal cells accelerate migration and activate AKT pathway in human keratinocytes and fibroblasts independently of miR-205 activity

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dc.creatorAndrea da Fonseca Ferreira
dc.creatorPricila da Silva Cunha
dc.creatorVirgínia Mendes Carregal
dc.creatorPriscila de Cássia da Silva
dc.creatorMarcelo Coutinho de Miranda
dc.creatorMarianna Kunrath-Lima
dc.creatorMariane Izabella Abreu de Melo
dc.creatorCamila Cristina Fraga Faraco
dc.creatorJoana Lobato Barbosa
dc.creatorFrédéric Frezard
dc.creatorVivian Resende
dc.creatorMichele Angela Rodrigues
dc.creatorAlfredo Miranda de Goes
dc.creatorDawidson Assis Gomes
dc.date.accessioned2023-07-18T16:41:17Z
dc.date.accessioned2025-09-08T23:10:26Z
dc.date.available2023-07-18T16:41:17Z
dc.date.issued2017
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1155/2017/9841035
dc.identifier.issn1687-9678
dc.identifier.urihttps://hdl.handle.net/1843/56578
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofStem Cells International
dc.rightsAcesso Aberto
dc.subjectCélulas-tronco mesenquimais
dc.subjectCélulas estromais
dc.subjectQueratinócitos
dc.subjectFibroblastos
dc.subject.otherMesenchymal stem
dc.subject.otherStromal Cells
dc.subject.otherKeratinocytes
dc.subject.otherFibroblasts
dc.titleExtracellular vesicles from adipose-derived mesenchymal stem/stromal cells accelerate migration and activate AKT pathway in human keratinocytes and fibroblasts independently of miR-205 activity
dc.typeArtigo de periódico
local.citation.volume2017
local.description.resumoMesenchymal stem/stromal cells (MSCs) are promising tools in cell therapy. They secrete extracellular vesicles (EVs) that carry different classes of molecules that can promote skin repair, but the mechanisms are poorly understood. Skin wound healing is a complex process that requires the activity of several signaling pathways and cell types, including keratinocytes and fibroblasts. In this study, we explored whether adipose tissue MSC-derived EVs could accelerate migration and proliferation of keratinocytes and fibroblasts, activate the AKT pathway, and promote wound healing in vivo. Furthermore, we evaluated if EV effects are miR-205 dependent. We found that MSC EVs had an average diameter of 135 nm. Keratinocytes and fibroblasts exposed to EVs exhibited higher levels of proliferation, migration, and AKT activation. Topical administration of EVs accelerated skin wound closure. Knockdown of miR-205 decreased AKT phosphorylation in fibroblasts and keratinocytes, whereas migration was decreased only in keratinocytes. Moreover, knockdown of miR-205 failed to inhibit AKT phosphorylation in fibroblasts and keratinocytes exposed to EVs. About the mechanism of EV effects, we found that incubation with EVs prevented inhibition of AKT activation by miR-205 knockdown, suggesting that EVs activate AKT independently of miR-205. In conclusion, we demonstrated that EVs are a promising tool for wound healing.
local.identifier.orcidhttps://orcid.org/0000-0002-4633-313X
local.identifier.orcidhttps://orcid.org/0000-0002-5012-7566
local.identifier.orcidhttps://orcid.org/0000-0002-1863-4963
local.identifier.orcidhttps://orcid.org/0000-0003-3783-5717
local.identifier.orcidhttps://orcid.org/0000-0003-4400-0427
local.identifier.orcidhttps://orcid.org/0000-0002-0233-7729
local.identifier.orcidhttps://orcid.org/0000-0002-0262-949X
local.identifier.orcidhttps://orcid.org/0000-0001-7714-991X
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE CIRURGIA
local.publisher.initialsUFMG
local.url.externahttps://www.hindawi.com/journals/sci/2017/9841035/

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