Detection of bacterial infection by a technetium-99m-labeled peptidoglycan aptamer

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dc.creatorIêda Mendes Ferreira
dc.creatorCamila Maria de Sousa Lacerda
dc.creatorSara Roberta dos Santos
dc.creatorAndré Luís Branco de Barros
dc.creatorSimone Odília Fernandes
dc.creatorValbert Nascimento Cardoso
dc.creatorAntero Silva Ribeiro de Andrade
dc.date.accessioned2022-05-11T00:26:39Z
dc.date.accessioned2025-09-08T23:49:45Z
dc.date.available2022-05-11T00:26:39Z
dc.date.issued2017-09
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.identifier.doi10.1016/j.biopha.2017.07.017
dc.identifier.issn0753-3322
dc.identifier.urihttps://hdl.handle.net/1843/41534
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBiomedicine & Pharmacotherapy
dc.rightsAcesso Restrito
dc.subjectRadiofármaco
dc.subjectInfecção bacteriana
dc.subject.otherAptamer
dc.subject.otherPeptidoglycan
dc.subject.otherTechnetium-99m
dc.subject.otherDiagnosis
dc.subject.otherBacterial infection
dc.subject.otherRadiopharmaceutical
dc.titleDetection of bacterial infection by a technetium-99m-labeled peptidoglycan aptamer
dc.typeArtigo de periódico
local.citation.epage938
local.citation.spage931
local.citation.volume93
local.description.resumoNuclear medicine clinicians are still waiting for the optimal scintigraphic imaging agents capable of distinguishing between infection and inflammation, and between fungal and bacterial infections. Aptamers have several properties that make them suitable for molecular imaging. In the present study, a peptidoglycan aptamer (Antibac1) was labeled with 99mTc and evaluated by biodistribution studies and scintigraphic imaging in infection‐bearing mice. Labeling with 99mTc was performed by the direct method and the complex stability was evaluated in saline, plasma and in the molar excess of cysteine. The biodistribution and scintigraphic imaging studies with the 99mTc-Antibac1 were carried out in two different experimental infection models: Bacterial-infected mice (S. aureus) and fungal-infected mice (C. albicans). A 99mTc radiolabeled library, consisting of oligonucleotides with random sequences, was used as a control for both models. Radiolabeling yields were superior to 90% and 99mTc‐Antibac1 was highly stable in presence of saline, plasma, and cysteine up to 6 h. Scintigraphic images of S. aureus infected mice at 1.5 and 3.0 h after 99mTc‐Antibac1 injection showed target to non-target ratios of 4.7 ± 0.9 and 4.6 ± 0.1, respectively. These values were statistically higher than those achieved for the 99mTc‐library at the same time frames (1.6 ± 0.4 and 1.7 ± 0.4, respectively). Noteworthy, 99mTc‐Antibac1 and 99mTc‐library showed similar low target to non-target ratios in the fungal-infected model: 2.0 ± 0.3 and 2.0 ± 0.6 for 99mTc‐Antibac1 and 2.1 ± 0.3 and 1.9 ± 0.6 for 99mTc‐library, at the same times. These findings suggest that the 99mTc‐Antibac1 is a feasible imaging probe to identify a bacterial infection focus. In addition, this radiolabeled aptamer seems to be suitable in distinguishing between bacterial and fungal infection.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0753332217326719?via%3Dihub

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