Efeito citotóxico do ibuprofeno e paracetamol sobre uma linhagem decélulas de câncer epitelial de ovário in vitro

Abstract

The purposes of this study was investigated whether low concentrations of acetaminophen (paracetamol) and ibuprofen inhibited the growth of this cell line in vitro and determined a basic expression of ABC transporters in an EOC cell line. TOV 21G cells were exposed todifferent concentrations of paracetamol (1.5 to 15 g/mL) and ibuprofen (2.0 to 20 g/mL) for 24 e 48 hours. Cellular growth was assessed using a cell viability assay. Cellular morphology was determined by fluorescence microscopy. The gene expression profile of ABC transporters was determined by assessing a panel of 42 genes of the ABC transporter superfamily. We observed a significant decrease in TOV 21G cell growth after exposure to 15 g/mL of paracetamol for 24 hours (p=0.02) and 48 hours (p=0.01) or to 20 g/mL of ibuprofen for 48hours (p=0.04). Assessing the morphology of TOV 21G cells did not reveal evidence of extensive apoptosis. TOV 21G cell shad reduced expression of the genes ABCA1, ABCC3, ABCC4, ABCD3, ABCD4 and ABCE1 within the ABC transporter superfamily. The current study provides in vitro evidence of growth inhibitory effects of therapeutic concentrations of paracetamol and ibuprofen on TOV 21G cells. Additionally, TOV 21G cells had reduced expression of the ABCA1, ABCC3, ABCC4, ABCD3, ABCD4 and ABCE1 transporters.