Amino acid biosignature in plasma among ischemic stroke subtypes

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dc.creatorVânia Aparecida Mendes Goulart
dc.creatorMarcelo Martins de Sena
dc.creatorThiago de Oliveira Mendes
dc.creatorHelvécio Costa Menezes
dc.creatorZenilda de Lourdes Cardeal
dc.creatorMaria José Nunes de Paiva
dc.creatorValéria Cristina Sandrim
dc.creatorMauro Cunha Xavier Pinto
dc.creatorRodrigo Ribeiro Resende
dc.date.accessioned2022-12-30T12:40:31Z
dc.date.accessioned2025-09-09T01:24:18Z
dc.date.available2022-12-30T12:40:31Z
dc.date.issued2019
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1155/2019/8480468
dc.identifier.issn2314-6133
dc.identifier.urihttps://hdl.handle.net/1843/48525
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBioMed Research International
dc.rightsAcesso Aberto
dc.subjectBioquímica
dc.subjectAminoácidos
dc.subjectMarcadores biológicos
dc.subjectAcidentes vasculares cerebrais
dc.subjectIsquemia cerebral
dc.subjectEspectrometria de massa
dc.subject.otherAmino acid
dc.subject.otherIschemic stroke
dc.subject.otherStroke etiology
dc.subject.otherMass spectrometry
dc.subject.otherGas chromatography
dc.subject.otherBiomarkers
dc.titleAmino acid biosignature in plasma among ischemic stroke subtypes
dc.typeArtigo de periódico
local.citation.epage11
local.citation.spage1
local.citation.volume2019
local.description.resumoIschemic stroke is a neurovascular disorder caused by reduced or blockage of blood flow to the brain, which may permanently affect motor and cognitive abilities. The diagnostic of stroke is performed using imaging technologies, clinical evaluation, and neuropsychological protocols, but no blood test is available yet. In this work, we analyzed amino acid concentrations in blood plasma from poststroke patients in order to identify differences that could characterize the stroke etiology. Plasma concentrations of sixteen amino acids from patients with chronic ischemic stroke (n = 73) and the control group (n = 16) were determined using gas chromatography coupled to mass spectrometry (GC-MS). The concentration data was processed by Partial Least Squares-Discriminant Analysis (PLS-DA) to classify patients with stroke and control. The amino acid analysis generated a first model able to discriminate ischemic stroke patients from control group. Proline was the most important amino acid for classification of the stroke samples in PLS-DA, followed by lysine, phenylalanine, leucine, and glycine, and while higher levels of methionine and alanine were mostly related to the control samples. The second model was able to discriminate the stroke subtypes like atherothrombotic etiology from cardioembolic and lacunar etiologies, with lysine, leucine, and cysteine plasmatic concentrations being the most important metabolites. Our results suggest an amino acid biosignature for patients with chronic stroke in plasma samples, which can be helpful in diagnosis, prognosis, and therapeutics of these patients.
local.identifier.orcidhttps://orcid.org/0000-0002-3234-8357
local.identifier.orcidhttps://orcid.org/0000-0001-5693-9015
local.identifier.orcidhttps://orcid.org/0000-0002-8346-1552
local.identifier.orcidhttps://orcid.org/0000-0002-5759-612X
local.identifier.orcidhttps://orcid.org/0000-0003-1383-6299
local.identifier.orcidhttps://orcid.org/0000-0002-6168-7470
local.identifier.orcidhttps://orcid.org/0000-0002-1680-8130
local.identifier.orcidhttps://orcid.org/0000-0002-2003-0739
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://www.hindawi.com/journals/bmri/2019/8480468/

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