DNA methylation of MMP9 is associated with high levels of MMP-9 messenger RNA in periapical inflammatory lesions

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dc.creatorKelma Campos
dc.creatorCarolina Cavalieri Gomes
dc.creatorLucyana Conceição Farias
dc.creatorRenato Menezes Silva
dc.creatorAriadne Letra
dc.creatorRicardo Santiago Gomez
dc.date.accessioned2025-06-06T21:15:29Z
dc.date.accessioned2025-09-08T22:48:34Z
dc.date.available2025-06-06T21:15:29Z
dc.date.issued2016-01
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.joen.2015.10.002
dc.identifier.issn1878-3554
dc.identifier.urihttps://hdl.handle.net/1843/82859
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Endodontics
dc.rightsAcesso Restrito
dc.subjectRNA, messenger
dc.subjectMatrix metalloproteinases
dc.subjectMethylation
dc.subjectRadicular cyst
dc.subjectTranscription, genetic
dc.subjectGenes
dc.subjectGene expression
dc.subjectGranuloma
dc.subject.otherMatrix metalloproteinase
dc.subject.otherMessenger RNA
dc.subject.otherMethylation
dc.subject.otherPeriapical lesion
dc.subject.otherRadicular cyst
dc.subject.otherTranscription
dc.titleDNA methylation of MMP9 is associated with high levels of MMP-9 messenger RNA in periapical inflammatory lesions
dc.typeArtigo de periódico
local.citation.epage130
local.citation.issue1
local.citation.spage127
local.citation.volume42
local.description.resumoIntroduction: Matrix metalloproteinases (MMPs) are the major class of enzymes responsible for degradation of extracellular matrix components and participate in the pathogenesis of periapical inflammatory lesions. MMP expression may be regulated by DNA methylation. The purpose of the present investigation was to analyze the expression of MMP2 and MMP9 in periapical granulomas and radicular cysts and to test the hypothesis that, in these lesions, their transcription may be modulated by DNA methylation. Methods: Methylation-specific polymerase chain reaction was used to evaluate the DNA methylation pattern of the MMP2 gene in 13 fresh periapical granuloma samples and 10 fresh radicular cyst samples. Restriction enzyme digestion was used to assess methylation of the MMP9 gene in 12 fresh periapical granuloma samples and 10 fresh radicular cyst samples. MMP2 and MMP9 messenger RNA transcript levels were measured by quantitative real-time polymerase chain reaction. Results: All periapical lesions and healthy mucosa samples showed partial methylation of the MMP2 gene; however, periapical granulomas showed higher MMP2 mRNA expression levels than healthy mucosa (P = .014). A higher unmethylated profile of the MMP9 gene was found in periapical granulomas and radicular cysts compared with healthy mucosa. In addition, higher MMP9 mRNA expression was observed in the periapical lesions compared with healthy tissues. Conclusions: The present study suggests that the unmethylated status of the MMP9 gene in periapical lesions may explain the observed up-regulation of messenger RNA transcription in these lesions.
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.identifier.orcidhttps://orcid.org/0000-0002-5451-140X
local.identifier.orcidhttps://orcid.org/0000-0002-7197-6735
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0099239915009000?via%3Dihub

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