Antifungal activity of eicosanoic acids isolated from the endophytic fungus Mycosphaerella sp. against Cryptococcus neoformans and C. gattii

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dc.creatorCristiane Bigatti Pereira
dc.creatorNívea Pereira de Sá
dc.creatorBeatriz Martins Borelli
dc.creatorCarlos Augusto Rosa
dc.creatorPaulo Jorge Sanches Barbeira
dc.creatorBetania Barros Cota
dc.creatorSusana Johann
dc.date.accessioned2024-04-18T13:50:32Z
dc.date.accessioned2025-09-09T00:43:48Z
dc.date.available2024-04-18T13:50:32Z
dc.date.issued2016
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.micpath.2016.09.022
dc.identifier.issn0882-4010
dc.identifier.urihttps://hdl.handle.net/1843/67389
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMicrobial Pathogenesis
dc.rightsAcesso Restrito
dc.subjectFungos - Biotecnologia
dc.subjectMembranas (Biologia)
dc.subjectFungos do solo
dc.subjectAtividade antifúngica
dc.subjectFungos patogênicos
dc.subjectTremellales
dc.subject.otherCryptococcus
dc.subject.otherMembrane damage
dc.subject.otherMycosphaerella
dc.subject.otherSynergism
dc.titleAntifungal activity of eicosanoic acids isolated from the endophytic fungus Mycosphaerella sp. against Cryptococcus neoformans and C. gattii
dc.typeArtigo de periódico
local.citation.epage212
local.citation.spage205
local.citation.volume100
local.description.resumoThe antifungal effects of two eicosanoic acids, 2-amino-3,4-dihydroxy-2-25-(hydroxymethyl)-14-oxo-6,12-eicosenoic acid (compound 1) and myriocin (compound 2), isolated from Mycosphaerella sp. were evaluated against Cryptococcus neoformans and C. gattii. The compounds displayed antifungal activities against several isolates of C. neoformans and C. gattii, with minimal inhibitory concentration (MIC) values ranging from 0.49 to 7.82 μM for compound 1 and 0.48-1.95 μM for compound 2. In the checkerboard microtiter test, both compounds exhibited synergistic activity with amphotericin B against C. gattii. Ultrastructural analysis revealed several signs of damage in C. gattii and C. neoformans cells treated with compounds 1 and 2, including deformities in cell shape, depressions on the surface, and withered cells. The cells of C. gattii treated with compounds 1 and 2 showed less loss of cellular material in comparison to those treated with amphotericin B. The difference in cellular material loss increased in a test compound concentration-dependent manner. Consistent with this observation, compounds 1 and 2 were able to internalize propidium iodide (PI) in C. gattii cells. In addition, compound 2 induced the formation of several pseudohyphae, suggesting that it could reduce virulence in C. gattii cells. The study results show that these natural products led to membrane damage; however, this may not be the main target of action. These compounds have potential antifungal activity and could be useful in further studies for developing more effective combination therapies with amphotericin B and reducing side effects in patients.
local.identifier.orcidhttps://orcid.org/0000-0002-9966-1321
local.identifier.orcidhttps://orcid.org/0000-0002-0056-9075
local.identifier.orcidhttps://orcid.org/0000-0002-0770-8680
local.identifier.orcidhttps://orcid.org/0000-0002-0041-2043
local.identifier.orcidhttps://orcid.org/0000-0001-8068-1720
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0882401016304740

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