Detecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion

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dc.creatorFernanda Ludolf
dc.creatorFernanda Fonseca Ramos
dc.creatorEduardo Antonio Ferraz Coelho
dc.creatorFlávia F. Bagno
dc.creatorJoão A. Oliveira-da-Silva
dc.creatorThiago A. R. Reis
dc.creatorMyron Christodoulides
dc.creatorPaula F. Vassallo
dc.creatorCecilia G. Ravetti
dc.creatorVandack Nobre
dc.creatorFlavio G. da Fonseca
dc.date.accessioned2023-11-06T20:47:11Z
dc.date.accessioned2025-09-09T01:20:46Z
dc.date.available2023-11-06T20:47:11Z
dc.date.issued2022-05-13
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1126/sciadv.abn7424
dc.identifier.issn2571-9262
dc.identifier.urihttps://hdl.handle.net/1843/60527
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofScience Advances
dc.rightsAcesso Aberto
dc.subjectImunologia
dc.subjectBiotecnologia
dc.subjectCOVID-19
dc.subjectUrina
dc.subjectDiagnóstico
dc.subject.otherDiagnóstico
dc.subject.otherCOVID-19
dc.subject.otherUrina
dc.titleDetecting anti–SARS-CoV-2 antibodies in urine samples: A noninvasive and sensitive way to assay COVID-19 immune conversion
dc.typeArtigo de periódico
local.citation.epage3
local.citation.issue19
local.citation.spageeabn7424
local.citation.volume8
local.description.resumoSerum-based ELISA (enzyme-linked immunosorbent assay) has been widely used to detect anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies. However, to date, no study has investigated patient urine as a biological sample to detect SARS-CoV-2 virus-specific antibodies. An in-house urine-based ELISA was developed using recombinant SARS-CoV-2 nucleocapsid protein. The presence of SARS-CoV-2 antibodies in urine was established, with 94% sensitivity and 100% specificity for the detection of anti–SARS-CoV-2 antibodies with the urine-based ELISA and 88% sensitivity and 100% specificity with a paired serum-based ELISA. The urine-based ELISA that detects anti–SARS-CoV-2 antibodies is a noninvasive method with potential application as a facile COVID-19 immunodiagnostic platform, which can be used to report the extent of exposure at the population level and/or to assess the risk of infection at the individual level.
local.identifier.orcidhttps://orcid.org/0000-0003-3357-9816
local.identifier.orcidhttps://orcid.org/0000-0002-7852-0300
local.identifier.orcidhttps://orcid.org/0000-0003-1578-6678
local.identifier.orcidhttps://orcid.org/0000-0002-9663-4731
local.identifier.orcidhttps://orcid.org/0000-0002-7531-0607
local.identifier.orcidhttps://orcid.org/0000-0002-7922-0422
local.identifier.orcidhttps://orcid.org/0000-0002-1416-8694
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.departmentMED - DEPARTAMENTO DE MEDICINA PREVENTIVA SOCIAL
local.publisher.departmentMEDICINA - FACULDADE DE MEDICINA
local.publisher.initialsUFMG
local.url.externahttps://www.science.org/doi/10.1126/sciadv.abn7424

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