Metformin treatment modulates long non-coding RNA isoforms expression in human cells

Página do item simplificado
dc.creatorIzabela Mamede Costa Andrade da Conceição
dc.creatorThomaz Lüscher Dias
dc.creatorLúcio Rezende Queiroz
dc.creatorAna Gabrielle Batista de Melo
dc.creatorCarlos Renato Machado
dc.creatorKarina Braga Gomes Borges
dc.creatorRenan Pedra de Souza
dc.creatorMarcelo Rizzatti Luizon
dc.creatorGlória Regina Franco
dc.date.accessioned2025-04-10T11:59:36Z
dc.date.accessioned2025-09-09T00:42:42Z
dc.date.available2025-04-10T11:59:36Z
dc.date.issued2022-10
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/ncrna8050068
dc.identifier.issn2311-553X
dc.identifier.urihttps://hdl.handle.net/1843/81436
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofNon-coding RNA
dc.rightsAcesso Aberto
dc.subjectRNA longo não codificante
dc.subjectMetformina
dc.subjectTranscriptoma
dc.subjectIsoformas de RNA
dc.subject.otherlncRNA
dc.subject.otherMetformin
dc.subject.otherTranscriptome
dc.subject.otherRNA isoforms
dc.titleMetformin treatment modulates long non-coding RNA isoforms expression in human cells
dc.typeArtigo de periódico
local.citation.epage14
local.citation.issue5
local.citation.spage1
local.citation.volume8
local.description.resumoLong noncoding RNAs (lncRNAs) undergo splicing and have multiple transcribed isoforms. Nevertheless, for lncRNAs, as well as for mRNA, measurements of expression are routinely performed only at the gene level. Metformin is the first-line oral therapy for type 2 diabetes mellitus and other metabolic diseases. However, its mechanism of action remains not thoroughly explained. Transcriptomic analyses using metformin in different cell types reveal that only protein-coding genes are considered. We aimed to characterize lncRNA isoforms that were differentially affected by metformin treatment on multiple human cell types (three cancer, two non-cancer) and to provide insights into the lncRNA regulation by this drug. We selected six series to perform a differential expression (DE) isoform analysis. We also inferred the biological roles for lncRNA DE isoforms using in silico tools. We found the same isoform of an lncRNA (AC016831.6-205) highly expressed in all six metformin series, which has a second exon putatively coding for a peptide with relevance to the drug action. Moreover, the other two lncRNA isoforms (ZBED5-AS1-207 and AC125807.2-201) may also behave as cis-regulatory elements to the expression of transcripts in their vicinity. Our results strongly reinforce the importance of considering DE isoforms of lncRNA for understanding metformin mechanisms at the molecular level.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2311-553X/8/5/68

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Metformin treatment modulates long non-coding RNA isoforms expression in human cells.pdf
Tamanho:
3.43 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG