Assessing pathogenic mutations in dental follicles as an attempt to identify early events in odontogenic tumours tumourigenesis

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dc.creatorBruna Pizziolo Coura
dc.creatorTaynara Asevedo Campos de Resende
dc.creatorVinícius César Barbosa de Menezes
dc.creatorVanessa Fátima Bernardes
dc.creatorSilvia Ferreira de Sousa
dc.creatorMarina Gonçalves Diniz
dc.creatorRicardo Santiago Gomez
dc.creatorCarolina Cavalieri Gomes
dc.date.accessioned2025-09-03T21:29:03Z
dc.date.accessioned2025-09-08T22:52:21Z
dc.date.available2025-09-03T21:29:03Z
dc.date.issued2020-05
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.archoralbio.2019.104523
dc.identifier.issn1879-1506
dc.identifier.urihttps://hdl.handle.net/1843/84828
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofArchives of Oral Biology
dc.rightsAcesso Restrito
dc.subjectNeoplasms
dc.subjectGenetics
dc.subjectMutation
dc.subjectOdontogenesis
dc.subjectOdontogenic tumors
dc.subjectAssociation
dc.subjectTooth, impacted
dc.subjectDental sac
dc.subjectEvaluation study
dc.subject.otherBenign tumour
dc.subject.otherGenetics
dc.subject.otherMutation
dc.subject.otherNormal tissue
dc.subject.otherOdontogenesis
dc.subject.otherOdontogenic tumour
dc.titleAssessing pathogenic mutations in dental follicles as an attempt to identify early events in odontogenic tumours tumourigenesis
dc.typeArtigo de periódico
local.citation.volume113
local.description.resumoObjective: Driver oncogenic mutations have been reported in several benign neoplasms. While ameloblastomas show BRAF p.V600E mutations, adenomatoid odontogenic tumours harbour either KRAS p.G12R or p.G12 V. The lack of understanding of the core molecular changes involved in tumour initiation and progression represents a critical barrier to developing new strategies for cancer detection and prevention. Considering the fact that ameloblastoma and adenomatoid odontogenic tumours can originate from dental follicles, we hypothesized that the BRAF and KRAS mutations might be early events in odontogenic tumours tumourigenesis. We aimed to assess BRAF and KRAS mutations in dental follicles associated with asymptomatic impacted teeth. Design: Forty-eight dental follicles containing odontogenic epithelial remnants were included in the study. As ameloblastomas most often occur in the posterior mandible and adenomatoid odontogenic tumours have a predilection for the anterior jaws, we assessed by allele-specific qPCR the presence of BRAF p.V600E in 32 dental follicles associated with impacted 3rd mandibular molar teeth and KRAS p.G12 V and KRAS p.G12R mutations in 16 dental follicle specimens obtained from around impacted anterior teeth. Sanger sequencing was used as an additional method. Results: None of the dental follicle cases tested positive for the mutations. Conclusion: In conclusion, we tried to detect the early genetic events associated with odontogenic tumours development in dental follicles, but we were unable to showcase that BRAF p.V600E and KRAS p.G12R or p.G12 V mutations are the early genetic events associated with odontogenic tumours development.
local.identifier.orcidhttps://orcid.org/0000-0002-9987-5311
local.identifier.orcidhttps://orcid.org/0000-0002-3983-2889
local.identifier.orcidhttps://orcid.org/0000-0002-0255-4977
local.identifier.orcidhttps://orcid.org/0000-0003-0194-7434
local.identifier.orcidhttps://orcid.org/0000-0001-7820-4749
local.identifier.orcidhttps://orcid.org/0000-0002-4212-1172
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0003996919302043?via%3Dihub

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