Uncovering a complex virome associated with the cacao pathogens ceratocystis cacaofunesta and ceratocystis fimbriata

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dc.creatorRoy Bogardid Ardónespinal
dc.creatorSabrina Ferreira de Santana
dc.creatorVinícius Castro Santos
dc.creatorGabriela Nicolle Ramos Lizardo
dc.creatorRaner José Santana Silva
dc.creatorRonan Xavier Corrêa
dc.creatorLeandro Lopes Loguercio
dc.creatorAristóteles Góes-Neto
dc.creatorCarlos Priminho Pirovani
dc.creatorPaula Luize Camargos Fonseca
dc.creatorEric Roberto Guimarães Rocha Aguiar
dc.date.accessioned2025-01-20T22:40:47Z
dc.date.accessioned2025-09-09T00:16:09Z
dc.date.available2025-01-20T22:40:47Z
dc.date.issued2023-02-09
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/pathogens12020287
dc.identifier.issn2076-0817
dc.identifier.urihttps://hdl.handle.net/1843/79343
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPathogens
dc.rightsAcesso Aberto
dc.subjectCacau
dc.subjectFungos
dc.subjectVírus
dc.subject.otherRNA interference
dc.subject.otherFungi
dc.subject.otherMycovirus
dc.subject.otherPhytopathogen
dc.subject.otherVirus
dc.titleUncovering a complex virome associated with the cacao pathogens ceratocystis cacaofunesta and ceratocystis fimbriata
dc.typeArtigo de periódico
local.citation.epage22
local.citation.issue287
local.citation.spage1
local.citation.volume12
local.description.resumoTheobroma cacao is one of the main crops of economic importance in the world as the source of raw material for producing chocolate and derivatives. The crop is the main source of income for thousands of small farmers, who produce more than 80% of the world's cocoa supply. However, the emergence, re-emergence and proliferation of pathogens, such as Ceratocystis spp., the causative agent of Ceratocystis wilt disease and canker disease, have been affecting the sustainability of many crops. Fungal control is laborious, often depending on fungicides that are expensive and/or toxic to humans, prompting researchers to look for new solutions to counteract the proliferation of these pathogens, including the use of biological agents such as mycoviruses. In this study, we investigated the diversity of microorganisms associated with the T. cacao pathogens Ceratocystis cacaofunesta and Ceratocystis fimbriata with a focus on the virome using RNA sequencing data available in public databases. We used a comprehensive bioinformatics pipeline containing several steps for viral sequence enrichment and took advantage of an integrated assembly step composed of different assemblers followed by sequence similarity searches using NCBI nonredundant databases. Our strategy was able to identify four putative C. cacaofunesta viruses (hypovirus, sclerotimonavirus, alphapartitivirus and narnavirus) and six C. fimbriata viruses (three alphaendornaviruses, one victorivirus and two mitoviruses). All the viral sequences identified showed similarity to viral genomes in public databases only at the amino acid level, likely representing new viral species. Of note, we present the first report of viruses associated with the cacao pathogens C. cacaofunesta and C. fimbriata and the second report of viral species infecting members of the Ceratocystidaceae family. Our findings highlight the need for further prospective studies to uncover the real diversity of fungus-infecting viruses that can contribute to the development of new management strategies.
local.identifier.orcidhttps://orcid.org/0000-0001-8871-2536
local.identifier.orcidhttps://orcid.org/0000-0002-7187-0877
local.identifier.orcidhttps://orcid.org/0000-0002-5418-7291
local.identifier.orcidhttps://orcid.org/0000-0003-2085-2836
local.identifier.orcidhttps://orcid.org/0000-0002-0805-2003
local.identifier.orcidhttps://orcid.org/0000-0002-4010-1565
local.identifier.orcidhttps://orcid.org/0000-0002-2326-2797
local.identifier.orcidhttps://orcid.org/0000-0002-7692-6243
local.identifier.orcidhttps://orcid.org/0000-0002-6176-4069
local.identifier.orcidhttps://orcid.org/0000-0002-8103-9235
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2076-0817/12/2/287

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