Alamandine induces neuroprotection in ischemic stroke models

Página do item simplificado
dc.creatorSthéfanie Chaves de Almeida Gonçalves
dc.creatorBeatriz Lopes Tecedor Bassi
dc.creatorLucas Miranda Kangussu
dc.creatorDaniele Teixeira Alves
dc.creatorLorena Kelly Santiago Ramos
dc.creatorLorena Figueiredo Fernandes
dc.creatorMarco Túllio Rodrigues Alves
dc.creatorRubén Dario Sinisterra Millán
dc.creatorGisele Eva Bruch
dc.creatorRobson Augusto Souza dos Santos
dc.creatorAndré Ricardo Massensini
dc.creatorMaria Jose Campagnole dos Santos
dc.date.accessioned2024-01-30T13:23:34Z
dc.date.accessioned2025-09-08T23:04:05Z
dc.date.available2024-01-30T13:23:34Z
dc.date.issued2022
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.2174/0929867329666220204145730
dc.identifier.issn1875-533X
dc.identifier.urihttps://hdl.handle.net/1843/63480
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofCurrent Medicinal Chemistry
dc.rightsAcesso Restrito
dc.subjectSistema renina-angiotensina
dc.subjectIsquemia cerebral
dc.subjectAcidentes vasculares cerebrais
dc.subjectCitocinas
dc.subjectImunofluorescência
dc.subject.otherRenin-angiotensin system
dc.subject.otherOxidative stress
dc.subject.otherBrain ischemia
dc.subject.otherOxygen and glucose deprivation
dc.subject.otherIntracerebroventricular
dc.subject.otherCytokines
dc.subject.otherNeurological deficit
dc.titleAlamandine induces neuroprotection in ischemic stroke models
dc.typeArtigo de periódico
local.citation.epage3498
local.citation.issue19
local.citation.spage3483
local.citation.volume29
local.description.resumoBackground and Objective: Stroke, a leading cause of mortality and disability, characterized by neuronal death, can be induced by a reduction or interruption of blood flow. In this study, the role of Alamandine, a new peptide of the renin-angiotensin system, was evaluated in in-vitro and in-vivo brain ischemia models. Methods: In the in-vitro model, hippocampal slices from male C57/Bl6 mice were placed in a glucose-free aCSF solution and bubbled with 95% N2 and 5% CO2 to mimic brain ischemia. An Alamandine concentration-response curve was generated to evaluate cell damage, glutamatergic excitotoxicity, and cell death. In the in-vivo model, cerebral ischemia/ reperfusion was induced by bilateral occlusion of common carotid arteries (BCCAo-untreated) in SD rats. An intracerebroventricular injection of Alamandine was given 20–30 min before BCCAo. Animals were subjected to neurological tests 24 h and 72 h after BCCAo. Cytokine levels, oxidative stress markers, and immunofluorescence were assessed in the brain 72 h after BCCAo. Results: Alamandine was able to protect brain slices from cellular damage, excitotoxicity and cell death. When the Alamandine receptor was blocked, protective effects were lost. ICV injection of Alamandine attenuated neurological deficits of animals subjected to BCCAo and reduced the number of apoptotic neurons/cells. Furthermore, Alamandine induced anti-inflammatory effects in BCCAo animals as shown by reductions in TNFα, IL- 1β, IL-6, and antioxidant effects through attenuation of the decreased SOD, catalase, and GSH activities in the brain. Conclusion: This study showed, for the first time, a neuroprotective role for Alamandine in different ischemic stroke models.
local.identifier.orcidhttps://orcid.org/0000-0003-2210-0902
local.identifier.orcidhttps://orcid.org/0009-0006-8742-7518
local.identifier.orcidhttps://orcid.org/0000-0003-3678-118X
local.identifier.orcidhttps://orcid.org/0000-0001-6114-9544
local.identifier.orcidhttps://orcid.org/0000-0002-4167-0049
local.identifier.orcidhttps://orcid.org/0000-0002-7300-6417
local.identifier.orcidhttps://orcid.org/0000-0001-7656-1849
local.identifier.orcidhttps://orcid.org/0000-0002-1508-3801
local.identifier.orcidhttps://orcid.org/0000-0001-9483-4206
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://www.eurekaselect.com/article/120687

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG