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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/40109
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dc.creatorCamila Henriques Coelhopt_BR
dc.creatorAdriana Oliveira Costapt_BR
dc.creatorAna Carolina Carvalho Silvapt_BR
dc.creatorMaíra Mazzoni Puccipt_BR
dc.creatorÂngela Vieira Serufopt_BR
dc.creatorHaendel Gonçalves Nogueira Oliveira Busattipt_BR
dc.creatorMaurício Duriganpt_BR
dc.creatorJonas Enrique Aguilar Peralespt_BR
dc.creatorDonat Alexander de Chapeaurougept_BR
dc.creatorDaniel Almeida da Silva e Silvapt_BR
dc.creatorMaria Aparecida Gomespt_BR
dc.creatorJuliano Simões Toledopt_BR
dc.creatorSteven M. Singerpt_BR
dc.creatorRosiane Aparecida da Silva Pereirapt_BR
dc.creatorAna Paula Salles Moura Fernandespt_BR
dc.date.accessioned2022-03-15T17:53:25Z-
dc.date.available2022-03-15T17:53:25Z-
dc.date.issued2016-
dc.citation.volume11pt_BR
dc.citation.issue10pt_BR
dc.citation.spage1pt_BR
dc.citation.epage15pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0164946pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/40109-
dc.description.resumoThe zoonotic potential of giardiasis, as proposed by WHO since the late 70's, has been largely confirmed in this century. The genetic assemblages A and B of Giardia duodenalis are frequently isolated from human and canine hosts. Most of the assemblage A strains are not infective to adult mice, which can limit the range of studies regarding to biology of G. duodenalis, including virulence factors and the interaction with host immune system. This study aimed to determine the infectivity in mice of an assemblage A Giardia duodenalis strain (BHFC1) isolated from a dog and to classify the strain in sub-assemblages (AI, AII, AIII) through the phylogenetic analysis of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. In addition, the proteomic profile of soluble and insoluble protein fractions of trophozoites was analyzed by 2D-electrophoresis. Accordingly, trophozoites of BHFC1 were highly infective to Swiss mice. The phylogenetic analysis of tpi and gdh revealed that BHFC1 clustered to sub-assemblage AI. The proteomic map of soluble and insoluble protein fractions led to the identification of 187 proteins of G. duodenalis, 27 of them corresponding to hypothetical proteins. Considering both soluble and soluble fractions, the vast majority of the identified proteins (n = 82) were classified as metabolic proteins, mainly associated with carbon and lipid metabolism, including 53 proteins with catalytic activity. Some of the identified proteins correspond to antigens while others can be correlated with virulence. Besides a significant complementation to the proteomic data of G. duodenalis, these data provide an important source of information for future studies on various aspects of the biology of this parasite, such as virulence factors and host and pathogen interactions.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofPlos Onept_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherGiardíasept_BR
dc.subject.otherGiardia lambliapt_BR
dc.subject.otherProteômicapt_BR
dc.subject.otherTrofozoítospt_BR
dc.subject.otherFilogeniapt_BR
dc.titleGenotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia duodenalis, infective to micept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0164946pt_BR
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