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http://hdl.handle.net/1843/41867| Type: | Artigo de Periódico |
| Title: | A simple and sensitive HPLC-FL method for simultaneous determination of angiotensin II receptor antagonists in human plasma |
| Authors: | Mateus Araújo Castro e Souza Naialy Fernandes Araújo Reis Isabella Marques da Silva Leite Maria Beatriz de Abreu Glória Gerson Antônio Pianetti Maria Auxiliadora Parreiras Martins Isabella Cristina Plácido de Oliveira Pacheco Christian Fernandes |
| Abstract: | Angiotensin II receptor antagonists are one of the most widely used classes of antihypertensive drugs. In this study, an HPLC fluorescence method after protein precipitation (PPT) extraction was developed and validated for determination of olmesartan, losartan, irbesartan, and valsartan in human plasma. The separation was carried out on a Luna cyano (250 × 4.6 mm i.d.; 5 μm particle size) column and the mobile phase was composed of acetonitrile and 0.1 % phosphoric acid in gradient elution, at a flow rate of 1.2 mL min−1. A PPT method was optimized by a two-level factorial design with triplicate at the central point. The parameters that could affect the extraction (sample volume and acetonitrile/plasma volume ratio) were evaluated and the method was compared to microextraction by packed sorbent (MEPS) and liquid-liquid extraction (LLE). The developed method allowed the simultaneous quantification of the analytes employing a simple and cheap sample preparation method and a short chromatographic run (13 min). This method was fully validated showing selectivity, precision, accuracy, and linearity over the range of 25.0–1500.0 ng mL−1 for olmesartan and valsartan, 25.0–2500.0 ng mL−1 for irbesartan, and 35.0–2500.0 ng mL−1 for losartan. Finally, the method was successfully applied in the analysis of human plasma from volunteers. |
| Subject: | Cromatografia líquida Anti-hipertensivos Método de fluorescência |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE ALIMENTOS FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1016/j.jpba.2020.113403 |
| URI: | http://hdl.handle.net/1843/41867 |
| Issue Date: | 5-Sep-2020 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0731708520312899 |
| metadata.dc.relation.ispartof: | Journal of Pharmaceutical and Biomedical Analysis |
| Appears in Collections: | Artigo de Periódico |
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