Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/41902| Type: | Artigo de Periódico |
| Title: | Vancomycin-loaded N,N-dodecyl,methyl-polyethylenimine nanoparticles coated with hyaluronic acid to treat bacterial endophthalmitis: development, characterization, and ocular biocompatibility |
| Authors: | Jéssica Ferreira Cardoso Fernanda Barçante Perasoli Tamires Cunha Almeida Maria Betânia de Freitas Marques Cibele Rodrigues Toledo Priscilla Oliveira Gil Harley da Silva Tavares Mariana Campos da Paz Wagner da Nova Mussel Juliana Teixeira Magalhães Glenda Nicioli da Silva Armando da Silva-Cunha Paulo Afonso Granjeiro Alexander M. Klibanov Gisele Rodrigues da Silva |
| Abstract: | Vancomycin-loaded N,N-dodecyl,methyl-polyethylenimine nanoparticles coated with hyaluronic acid (VCM-DMPEI nanoparticles/HA) were synthesized as an adjuvant for the treatment of bacterial endophthalmitis. The nanoparticles were formulated by experimental statistical design, thoroughly characterized, and evaluated in terms of bactericidal activity and both in vitro and in vivo ocular biocompatibility. The VCM-DMPEI nanoparticles/HA were 154 ± 3 nm in diameter with a 0.197 ± 0.020 polydispersity index; had a + 26.4 ± 3.3 mV zeta potential; exhibited a 93% VCM encapsulation efficiency; and released 58% of the encapsulated VCM over 96 h. VCM and DMPEI exhibited a synergistic bactericidal effect. The VCM-DMPEI nanoparticles/HA were neither toxic to ARPE-19 cells nor irritating to the chorioallantoic membrane. Moreover, the VCM-DMPEI nanoparticles/HA did not induce modifications in retinal functions, as determined by electroretinography, and in the morphology of the ocular tissues. In conclusion, the VCM-DMPEI nanoparticles/HA may be a useful therapeutic adjuvant to treat bacterial endophthalmitis. |
| Subject: | Endoftalmite bacteriana Vancomicina Ácido hialurônico |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE ALIMENTOS ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1016/j.ijbiomac.2020.12.057 |
| URI: | http://hdl.handle.net/1843/41902 |
| Issue Date: | 1-Feb-2021 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0141813020352156#! |
| metadata.dc.relation.ispartof: | International Journal of Biological Macromolecules |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.