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http://hdl.handle.net/1843/42419| Type: | Artigo de Periódico |
| Title: | Multi-walled carbon nanotubes functionalized with recombinant Dengue virus 3 envelope proteins induce significant and specific immune responses in mice |
| Authors: | Alice Freitas Versiani Ado Jorio de Vasconcelos Flávio Guimarães da Fonseca Ruiz Gerhardt Astigarraga Eliseu Soares de Oliveira Rocha Ana Paula Moreira Barboza Erna Geessien Kroon Milene Alvarenga Rachid Daniele da Glória de Souza Luiz Orlando Ladeira Edel Figueiredo Barbosa Stancioli |
| Abstract: | Background: Dengue is the most prevalent arthropod‑borne viral disease in the world. In this article we present results on the development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. Methods: The MWNT‑DENV3E nanoconjugate was developed by covalent functionalization of carboxylated multi‑walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. The recombinant antigens were bound to the MWNT using a diimide‑activated amidation process and the immunogen was characterized by TEM, AFM and Raman Spectroscopy. Furthermore, the immunogenicity of this vaccine candidate was evaluated in a murine model. Results: Immunization with MWNT‑DENV3E induced comparable IgG responses in relation to the immunization with non‑conjugated proteins; however, the inoculation of the nanoconjugate into mice generated higher titers of neutralizing antibodies. Cell‑mediated responses were also evaluated, and higher dengue‑specific splenocyte proliferation was observed in cell cultures derived from mice immunized with MWNT‑DENV3E when compared to animals immu‑ nized with the non‑conjugated DENV3E. Conclusions: Despite the recent licensure of the CYD‑TDV dengue vaccine in some countries, results from the vaccine’s phase III trial have cast doubts about its overall efficacy and global applicability. While questions about the effectiveness of the CYD‑TDV vaccine still lingers, it is wise to keep at hand an array of vaccine candidates, including alternative non‑classical approaches like the one presented here. |
| Subject: | Nanotubos de carbono Espectroscopia de Raman Dengue |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE MICROBIOLOGIA ICB - DEPARTAMENTO DE PATOLOGIA ICX - DEPARTAMENTO DE FÍSICA VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1186/s12951-017-0259-4 |
| URI: | http://hdl.handle.net/1843/42419 |
| Issue Date: | 4-Apr-2017 |
| metadata.dc.url.externa: | https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-017-0259-4 |
| metadata.dc.relation.ispartof: | Journal of Nanobiotechnology |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Multi-walled carbon nanotubes.pdf | 7.24 MB | Adobe PDF | View/Open |
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