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http://hdl.handle.net/1843/42608| Tipo: | Artigo de Periódico |
| Título: | Total combining power: technique for the evaluation of the quality control process of clostridiosis vaccines |
| Título(s) alternativo(s): | Poder total de combinação: Técnica para avaliação do processo de controle de qualidade de vacinas contra clostridiose |
| Autor(es): | Igor Viana Brandi Eliane Macedo Sobrinho Santos Bruna Mara Aparecida de Carvalho Mesquita Carla Adriana Ferreira Durães Paula Karoline Soares Farias Roger Shoji Sari Alex Sander Rodrigues Cangussu Adalberto Pessoa |
| Resumo: | An efficient technique for evaluation of the quality control of vaccines against clostridiosis is described in this study. This technique is capable of quantifying the toxoid of the bacterium Clostridium perfringens Type D, which is commonly found within these vaccines. The described method is performed in vivo to quantify the toxoid, replacing the current predominant approaches that use the titration of toxins before the inactivation process. This method is based on the partial neutralization of a determined dose of antitoxin by testing different doses of the toxoid. In order to guarantee its reliability, it is essential for the technique to be validated. Thus, the technique was tested using the following validation parameters: specificity and selectivity, detection limit, linear correlation, precision and robustness, in agreement with the requirements of regulatory agencies and international committees from around the world. The method was found to be specific, selective, robust, precise, and linear inside a specific concentration range. Therefore, it could be applied to the quality control of clostridiosis vaccines with satisfactory results. |
| Assunto: | Vacina veterinária Clostridioses Toxinas bacterianas |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Tipo de Acesso: | Acesso Aberto |
| Identificador DOI: | https://doi.org/10.1016/j.mimet.2016.08.023 |
| URI: | http://hdl.handle.net/1843/42608 |
| Data do documento: | 2016 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0167701216302330 |
| metadata.dc.relation.ispartof: | Journal of Microbiological Methods |
| Aparece nas coleções: | Artigo de Periódico |
Arquivos associados a este item:
| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Total combining power technique for the evaluation of the quality control process of clostridiosis vaccines.pdf | 260.39 kB | Adobe PDF | Visualizar/Abrir |
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