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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/42608
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dc.creatorIgor Viana Brandipt_BR
dc.creatorEliane Macedo Sobrinho Santospt_BR
dc.creatorBruna Mara Aparecida de Carvalho Mesquitapt_BR
dc.creatorCarla Adriana Ferreira Durãespt_BR
dc.creatorPaula Karoline Soares Fariaspt_BR
dc.creatorRoger Shoji Saript_BR
dc.creatorAlex Sander Rodrigues Cangussupt_BR
dc.creatorAdalberto Pessoapt_BR
dc.date.accessioned2022-06-23T11:14:04Z-
dc.date.available2022-06-23T11:14:04Z-
dc.date.issued2016-
dc.citation.volume130pt_BR
dc.citation.spage164pt_BR
dc.citation.epage168pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.mimet.2016.08.023pt_BR
dc.identifier.issn0167-7012pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/42608-
dc.description.resumoAn efficient technique for evaluation of the quality control of vaccines against clostridiosis is described in this study. This technique is capable of quantifying the toxoid of the bacterium Clostridium perfringens Type D, which is commonly found within these vaccines. The described method is performed in vivo to quantify the toxoid, replacing the current predominant approaches that use the titration of toxins before the inactivation process. This method is based on the partial neutralization of a determined dose of antitoxin by testing different doses of the toxoid. In order to guarantee its reliability, it is essential for the technique to be validated. Thus, the technique was tested using the following validation parameters: specificity and selectivity, detection limit, linear correlation, precision and robustness, in agreement with the requirements of regulatory agencies and international committees from around the world. The method was found to be specific, selective, robust, precise, and linear inside a specific concentration range. Therefore, it could be applied to the quality control of clostridiosis vaccines with satisfactory results.pt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Microbiological Methodspt_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherVacina veterináriapt_BR
dc.subject.otherClostridiosespt_BR
dc.subject.otherToxinas bacterianaspt_BR
dc.titleTotal combining power: technique for the evaluation of the quality control process of clostridiosis vaccinespt_BR
dc.title.alternativePoder total de combinação: Técnica para avaliação do processo de controle de qualidade de vacinas contra clostridiosept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0167701216302330pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6714-7996pt_BR
dc.identifier.orcidhttps://orcid.org/ 0000-0001-8980-8599pt_BR
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