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http://hdl.handle.net/1843/44385| Type: | Artigo de Periódico |
| Title: | Leptin impairs the therapeutic effect of ionizing radiation inoral squamous cell carcinoma cells |
| Authors: | Rogério Gonçalves da Rocha Eliane Macedo Sobrinho Santos Eloá Mangabeira Santos Emisael Stênio Batista Gomes Guilherme Veloso Ramos Karina Marini Aguiar Bruno Rodrigues Gonçalves Sérgio Henrique Sousa Santos Alfredo Maurício Batista de Paula André Luiz Sena Guimarães Lucyana Conceição Farias |
| Abstract: | Purpose: leptin, an important hormone controlling energy homeostasis, has been linked to the pathogenesis of oral squamous cell carcinoma (OSCC). Evidence indicates that head and neck cancer patients undergoing radiotherapy show decreased leptin levels after radiotherapy treatment. Thus, we investigated, through phenotypic and molecular analyses, whether leptin can compromise the therapeutic effect of ionizing radiation and neoplastic behavior of OSCC cells. Methods: the human OSCC-derived cell lines SCC9 and SCC4 were treated with human recombinant leptin and exposed to 6 Gy of irradiation. We performed the in vitro assays of cell migration, death, proliferation, and colony-forming ability. The reactive oxygen species (ROS) levels and proteome analysis by mass spectrometry were also conducted. Results: leptin was able to increase cell proliferation, migration, and colony-forming ability, despite the suppressive effect induced by irradiation. Furthermore, the leptin promoted a significant reduction of ROS intracellular accumulation, and increased expression of the cancer-related proteins, as ACTC1, KRT6A, and EEF2 in irradiated OSCC cells. Conclusions: our findings suggest that leptin impairs responsivity of OSCC cells to the ionizing radiation, reducing the suppressive effects of irradiation on the neoplastic phenotype, and increasing protein expression critical to carcinogenesis. |
| Subject: | Câncer Radiação ionizante Homeostase Células - Proliferação Carcinogênese |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/jop.12786 |
| URI: | http://hdl.handle.net/1843/44385 |
| Issue Date: | Jan-2019 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/jop.12786 |
| metadata.dc.relation.ispartof: | Journal of Oral Pathology and Medicine |
| Appears in Collections: | Artigo de Periódico |
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