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http://hdl.handle.net/1843/44672| Type: | Artigo de Periódico |
| Title: | Modulation of streptococcus mutans virulence by dental adhesives containing anti-caries agents |
| Authors: | Carolina Bosso André Pedro Luiz Rosalen Lívia Câmara de Carvalho Galvão Bruna Marin Fronza Glaucia Maria Bovi Ambrosano Jack Liborio Ferracane Marcelo Giannini |
| Abstract: | Objectives: The aim of this in vitro study was to analyze the effect of the incorporation of two anti-caries agents into dental adhesives on the reduction of the virulence of Streptococcus mutans and on the adhesion to dentin. Methods: Apigenin (1mM) and tt-Farnesol (5mM) were added separately and in combination to a self-etch adhesive (CS3 - Clearfil S3 Bond Plus) and to an each-and-rinse adhesive (OPT - OptiBond S). Biofilm of S. mutans was grown on adhesive-coated hydroxyapatite disks for 115h and bacterial viability, dry-weight, alkali soluble, water soluble, intracellular polysaccharides and protein were quantified. Bond strength and dentin-adhesive interface were performed to analyze the effects of the incorporation on the physical properties and to identify changes in hybrid layer morphology. Results: Addition of Apigenin and Apigenin+tt-Farnesol to CS3, and Apigenin or tt-Farnesol to OPT reduced the dry-weight of S. mutans biofilm. Insoluble polysaccharide decreased with the addition of Apigenin to CS3 and tt-Farnesol to OPT. Intracellular polysaccharide decreased with addition of Apigenin and Apigenin+tt-Farnesol to CS3. No changes in dentin bond strength, resin-dentin interfacial morphology, total amount of protein and soluble polysaccharide were observed with the additions. Significance: Biofilms that are less cariogenic around dental restorations could decrease secondary caries formation; in addition, the reduction of virulence of S. mutans without necessarily killing the microorganism is more unlikely to induce antimicrobial resistance. |
| Subject: | Apigenin Dental cements Dental caries Streptococcus mutans |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1016/j.dental.2017.07.006 |
| URI: | http://hdl.handle.net/1843/44672 |
| Issue Date: | Oct-2017 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0109564117300556?via%3Dihub |
| metadata.dc.relation.ispartof: | Dental Materials |
| Appears in Collections: | Artigo de Periódico |
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