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Type:	Artigo de Periódico
Title:	Immunological profile of teeth with inflammatory periapical disease from chronic liver disease patients
Authors:	Julia Mourão Braga Marcela Carvalho Espaladori Maria Elisa de Souza e Silva Luciana Carla Brito Neves Leda Quercia Vieira Antônio Paulino Ribeiro Sobrinho
Abstract:	Aim To evaluate the mRNA expression levels of the cytokines interferon-γ, tumour necrosis factor-α, interleukin (IL)-1β, IL-10, IL-6, VEGF, and AGT and the chemokine CCL2/MCP-1 in periapical interstitial fluid associated with root canal infections before and after the reduction of the bacterial load using a cleaning procedure. Methodology The case group included 11 patients with chronic liver disease, and the control group included 11 healthy patients. Clinical samples were taken from teeth with pulp necrosis. After cleaning and drying the canal, three paper points were introduced into the root canal and passed through the root apex (2 mm) into the periapical tissues for 1 min. The samples were collected immediately after root canal cleaning and 7 days later to characterize those gene expression levels using real-time PCR. The data were subjected to the Shapiro–Wilk and the Wilcoxon tests. Results In the control group, significantly increased expression of the pro-inflammatory cytokines IFN-γ and TNF-α was observed in teeth with restrained bacterial loads (day 7) (P < 0.05). Similarly, increased TNF-α expression was found on day 7 in the liver group (P < 0.05). No differences were observed in the expression levels of the IL-1β, IL-10 and, IL-6, MCP-1/CCL-2 and VEGF between the first collection (day 0) and second collection (day 7), over time in either group. Conclusion Chronic liver disease patients exhibited sufficient immunologic ability showing relatively similar expression levels of cytokines, chemokines and angiogenic factors in periapical samples compared with the responses from no-chronic liver disease patients. The outcomes of this study suggest that liver impairment did not compromise the periapical immune response
Subject:	RNA messenger Cytokines Infections Dental pulp cavity
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.1111/iej.12992
URI:	http://hdl.handle.net/1843/45444
Issue Date:	8-Aug-2018
metadata.dc.url.externa:	https://onlinelibrary.wiley.com/doi/10.1111/iej.12992
metadata.dc.relation.ispartof:	International Endodontic Journal
Appears in Collections:	Artigo de Periódico

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