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http://hdl.handle.net/1843/45481| Type: | Artigo de Periódico |
| Title: | Concentration-dependent effect of bleaching agents on the immunolabeling of interleukin-6, interleukin-17, and cd5-positive cells in the dental pulp |
| Authors: | Francine Benetti João Eduardo Gomes Filho Ferreira Gustavo Sivieri de Araújo Edilson Ervolino André Luiz Fraga Briso Luciano Tavares Angelo Cintra |
| Abstract: | Aim: To evaluate lymphocyte-like cell activation (CD5-positive cells) and the expression of interleukin (IL)-6 and IL-17 in the pulp after tooth bleaching with two concentrations of hydrogen peroxide (H2 O2 ). Methodology: The right and left maxillary molars from 40 rats were treated randomly with bleaching gel with 20% H2 O2 (BLUE group, 1 application of 50 min), 35% H2 O2 (MAXX group, three applications of 15 min), or placebo gel (control). After 2 and 30 days, the rats were killed (n = 10), and the jaws were processed for histological and immunohistochemistry analysis of the pulp tissue. The scores of inflammation and immunolabelling (IL-6/IL-17) were submitted to Mann-Whitney and Kruskal-Wallis followed Dunn tests, respectively; anova tests were used for comparisons of number of CD5-positive cells and pulp chamber area values (P < 0.05). Results: At 2 days, 60% of specimens of the BLUE group were associated with moderate inflammation in pulp horns, and in the MAXX group with necrosis (P < 0.05). At 30 days, the pulp was organized, and tertiary dentine was formed. The MAXX group had superior immunolabelling of IL-17 at 2 days differing significantly from other groups (P < 0.05). At 2 days, 90% of the specimens of the BLUE group had moderate immunolabelling of IL-6, and 50% of the MAXX group had severe immunolabelling, both significantly different from the control (P < 0.05). There was no significant difference between the groups at 30 days (P > 0.05). CD5-positive cells were present at 2 and 30 days, particularly in the bleached groups (P < 0.05), without significant difference between time periods (P > 0.05). Conclusions: IL-6 and IL-17 participated in inflammation in the pulp tissue of rats after tooth bleaching, particularly at 2 days. The immunolabelling was greater with increasing H2 O2 concentration. This process was accompanied by the prolonged activation of CD5-positive cells. |
| Subject: | Tooth bleaching Interleukin-17 Interleukin-6 Dental pulp cavity Hydrogen peroxide |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | 10.1111/iej.12891 |
| URI: | http://hdl.handle.net/1843/45481 |
| Issue Date: | 20-Jan-2018 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/iej.12891 |
| metadata.dc.relation.ispartof: | International Endodontic Journal |
| Appears in Collections: | Artigo de Periódico |
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