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http://hdl.handle.net/1843/45699| Type: | Artigo de Periódico |
| Title: | Presence of osteocalcin, osteopontin, and reactive oxygen species-positive cells in pulp tissue after dental bleaching |
| Authors: | Francine Benetti André Luiz Fraga Briso Marina Carminatti de Araújo Lopes Barbosa Edilson Ervolino João Eduardo Gomes Filho Luciano Tavares Angelo Cintra |
| Abstract: | Aim: To analyse the influence of H2 O2 on pulp repair through osteocalcin and osteopontin immunolabelling and in cellular defence by using the antireactive oxygen species (ROS) antibody. Methodology: The maxillary molars of 50 rats were treated with 35% H2 O2 (Ble groups) or placebo gel (control groups). At 0 h and 2, 7, 15 and 30 days (n = 10 hemimaxillae), the rats were killed and pulp tissue was evaluated using inflammation and immunolabelling scores (osteocalcin/osteopontin); ROS-positive cells were counted. Paired t-test and Wilcoxon signed-rank test were used (P < 0.05). Results: The Ble group had necrosis in the coronal pulp at 0 h and in the occlusal third of the coronal pulp at 2 days; at 7, 15 and 30 days, no inflammation was noted similar to the controls (P > 0.05). Osteocalcin was absent in the Ble at 0 h, moderate at 2 days and increased thereafter, differing from the controls at all two periods (P < 0.05). Osteopontin was higher principally at 7 and 15 days in Ble groups, but differing with control groups from 2 days after bleaching (P < 0.05). The Ble group had more ROS-positive cells in the pulp at 7 and 15 days (P < 0.05). Tertiary dentine was observed at 7 days, increasing thereafter (P < 0.05). Conclusions: Post-bleaching pulp repair was associated with increased osteocalcin over time. Osteopontin also participated in this process, and anti-ROS was involved in cellular defence against H2 O2 . |
| Subject: | Hydrogen peroxide Osteocalcin Osteopontin Antibodies Inflammation |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/iej.13049 |
| URI: | http://hdl.handle.net/1843/45699 |
| Issue Date: | 2019 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/iej.13049 |
| metadata.dc.relation.ispartof: | International Endodontic Journal |
| Appears in Collections: | Artigo de Periódico |
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