Please use this identifier to cite or link to this item:
http://hdl.handle.net/1843/46093| Type: | Artigo de Periódico |
| Title: | Auxin production by endophytic bacteria isolated from banana trees |
| Authors: | Izabela Cristina Pires Gomes Amanda Dayane Malta Matos Silvia Nietsche Adelica Aparecida Xavier Márcia Regina Costa Wellington Silva Gomes Marlon Cristian Toledo Pereira |
| Abstract: | This study aimed to evaluate the capacity for in vitro indole compound synthesis by endophytic bacteria and tocompare the Salkowski colorimetric and high-performance liquid chromatography (HPLC) quantification methods. Initial screenings were performed using 10% tryptic soy agar (TSA) medium with and without L-tryptophan via theSalkowski colorimetric method in a completely randomized design with 40 treatments and three replications. In thesecond experiment, the five most promising isolates were used for the detection and quantification of indoles by HPLC in a completely randomized design with five treatments and three replications. Of the 40 isolates, 16synthesized indole-3-acetic acid (IAA) in the presence of L-tryptophan, and only three synthesized IAA in theabsence of this amino acid. The concentration of IAA determined by absorbance indicated that Agrobacteriumtumefaciens isolate EB.07 showed a higher concentration of IAA in the presence of L-tryptophan (16.61 μ g mL -1 ). HPLC analysis revealed the production of the following four indole compounds by these bacteria: IAA, indole pyruvic acid (IPA), indole lactic acid (ILA), and indole-3-acetaldehyde (IAAld). Significant differences between thetwo estimation methods were observed for two of the five tested isolates: A. tumefaciens (EB.07) and Bacillus pumilus (EB.12). For both isolates, the Salkowski method indicated higher concentrations than those obtained with HPLC. Considering the obtained results, the Salkowski colorimetric method should be used in initial screenings,and HPLC should be used as a complementary method in the selection of isolates with the highest potential tosynthesize indoles. |
| Subject: | Plantas - Efeito da auxina Alcalóides indólicos |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | 10.1590/1678-4324-2017160484 |
| URI: | http://hdl.handle.net/1843/46093 |
| Issue Date: | 2017 |
| metadata.dc.relation.ispartof: | Brazilian Archives of Biology and Technology |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Auxin production by endophytic bacteria isolated from banana trees.pdf | 1.14 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.