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http://hdl.handle.net/1843/46256
Type: | Artigo de Periódico |
Title: | An accurate identification of Pythium insidiosum complex using MALDI-TOF, requires representative spectra of each of its four phylogenetic clusters |
Authors: | Leonel Mendoza Raquel Virgínia Rocha Vilela Rinosh Mani |
Abstract: | On June 15 2019, a case report appeared in the online edition of International Journal of Infectious Diseases (IJID) by Bernheim et al., 2019. In the report, the authors described the management of a keratitis case caused by Pythium insidiosum in a man from Spain. The diagnosis was made through molecular methodologies, but no DNA sequences were deposited at GenBank. The isolation of “a large mycelium” on Brain-Heart-Infusion (BHI) was mentioned, but the isolate was not deposited at a culture collection. Although an eye biopsy using pan-fungal primers identified the organism in the biopsied tissue as P. insidiosum, there was not a description on how the isolate recovered from BHI was finally identified as P. insidiosum. |
Abstract: | Em 15 de junho de 2019, um relato de caso apareceu na edição online do International Journal of Infectious Diseases (IJID) por Bernheim et al., 2019. No relatório, os autores descreveram o manejo de um caso de ceratite causada por Pythium insidiosum em um homem da Espanha. O diagnóstico foi feito por meio de metodologias moleculares, mas nenhuma sequência de DNA foi depositada no GenBank. O isolamento de “um grande micélio” em Brain-Heart-Infusion (BHI) foi mencionado, mas o isolado não foi depositado em uma coleção de cultura. Embora uma biópsia ocular usando primers pan-fúngicos tenha identificado o organismo no tecido biopsiado como P. insidiosum, não havia uma descrição de como o isolado recuperado do BHI foi finalmente identificado como P. insidiosum. |
Subject: | Ceratite Pythium Filogenia |
language: | eng |
metadata.dc.publisher.country: | Brasil |
Publisher: | Universidade Federal de Minas Gerais |
Publisher Initials: | UFMG |
metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS |
Rights: | Acesso Aberto |
metadata.dc.identifier.doi: | https://doi.org/10.1016/j.ijid.2019.07.025 |
URI: | http://hdl.handle.net/1843/46256 |
Issue Date: | 1-Oct-2019 |
metadata.dc.url.externa: | https://www.ijidonline.com/article/S1201-9712(19)30310-8/fulltext#relatedArticles |
metadata.dc.relation.ispartof: | International Journal of Infectious Diseases |
Appears in Collections: | Artigo de Periódico |
Files in This Item:
File | Description | Size | Format | |
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An accurate identification of Pythium insidiosum complex using MALDI-TOF, requires representative spectra of each of its four phylogenetic clusters.pdf | 239.29 kB | Adobe PDF | View/Open |
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