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http://hdl.handle.net/1843/47905| Type: | Artigo de Periódico |
| Title: | Inactivated alpha toxin from Clostridium novyi type B in nano-emulsion protect partially protects Swiss mice from lethal alpha toxin challenge |
| Authors: | Mellanie Karoline do Carmo Félix Túllio Teixeira Deusdará Lucas Samuel Soares dos Santos Raimundo Wagner de Souza Aguiar Roberto Franco Teixeira Corrêa Igor Viana Brandi Eliane Macedo Sobrinho Bergmann Morais Ribeiro Luis André Morais Mariúba Paulo Afonso Nogueira Kattyanne Souza Costa Kelvinson Fernandes Viana Alex Sander Rodrigues Cangussu |
| Abstract: | Nano-emulsions are promising carriers for antigen delivery. Here, we evaluated the efficacy of a water-oil nano-emulsion containing concentrated, inactivated Clostridium novyi (C. novyi) type B supernatant culture (nano-iCnB) in protecting Swiss mice against a lethal dose of alpha toxin concentrated extract. Proteins were confirmed in the nano-iCnB and their stabilities were determined according physical parameters such as Zeta Potential (ZP). Biochemical, hematological parameters and morphological appearance of liver, spleen and thigh muscle alterations were examined to determine the safety of the compound. Partial protection against lethal doses was achieved in immunized mice despite low IgG titers. These data suggest that our nano-emulsion is a simple and efficient method of promoting antigen delivery for toxin-related diseases. |
| Subject: | Adjuvantes imunológicos Clostridium Antígenos |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | 10.1038/s41598-019-50683-2 |
| URI: | http://hdl.handle.net/1843/47905 |
| Issue Date: | 1-Oct-2019 |
| metadata.dc.url.externa: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6773766/ |
| metadata.dc.relation.ispartof: | Scientific Reports |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Inactivated alpha toxin from clostridium novyi type b in nano-emulsion protect partially protects swiss mice from lethal alpha toxin challenge.pdf | 1.27 MB | Adobe PDF | View/Open |
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