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http://hdl.handle.net/1843/48526| Type: | Artigo de Periódico |
| Title: | Determination of amino acids in plasma samples of preeclampsia patients by liquid chromatography coupled to high-resolution mass spectrometry |
| Authors: | Eliane do Nascimento Helvécio Costa Menezes Rodrigo Ribeiro Resende Vânia Aparecida Mendes Goulart Zenilda de Lourdes Cardeal |
| Abstract: | This work presents the application of a simple and sensitive method developed for the simultaneous analysis of the levels of twenty two underivatizated free L-amino acids in plasma samples of preeclampsia patients by high performance liquid chromatography coupled to high-resolution mass spectrometer (LC-HRMS) using hydrophilic interaction liquid chromatography (HILIC) silica column. The linearity was evaluated in the range of 0.009 to 8.018 μmol L-¹ showing coefficients of determination (R²) in the range of 0.999 to 0.911 (p < 0.05). The intra- and inter-day precisions were above of 9.84 and 15.31, respectively. The limits of detection (LOD) and quantification (LOQ) were in the range of 0.003 to 0.683 μmol L-¹ and 0.009 to 2.276 μmol L-¹, respectively. The results of the investigation of free L-amino acids showed that the levels of GABA, Arg, Ser, Leu, Ile, Met, Val, Ala, Gly, Bet, His and Lys in plasma of patients with preeclampsia were significantly different from healthy patients. |
| Subject: | Química bioinorganica Bioquímica Química inorgânica Aminoácidos Marcadores biológicos Pre-eclampsia Cromatografia liquida Espectrometria de massa |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICX - DEPARTAMENTO DE QUÍMICA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.21577/0103-5053.20190093 |
| URI: | http://hdl.handle.net/1843/48526 |
| Issue Date: | 2019 |
| metadata.dc.url.externa: | http://static.sites.sbq.org.br/jbcs.sbq.org.br/pdf/2019-0048AR_BrazWoman.pdf |
| metadata.dc.relation.ispartof: | Journal of the Brazilian Chemical Society |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Determination of amino acids in plasma samples of preeclampsia patients by liquid chromatography coupled to high-resolution mass spectrometry.pdf | 612.87 kB | Adobe PDF | View/Open |
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