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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/49151
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dc.creatorVivianne Carvalho da Cunha Trajanopt_BR
dc.creatorMaria Esperanza Cortés Segurapt_BR
dc.creatorRuben Dario Sinisterrapt_BR
dc.creatorCélia Regina Lanzapt_BR
dc.creatorCostapt_BR
dc.date.accessioned2023-01-25T21:42:10Z-
dc.date.available2023-01-25T21:42:10Z-
dc.date.issued2016-07-
dc.citation.volume64pt_BR
dc.citation.spage370pt_BR
dc.citation.epage375pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.msec.2016.03.103pt_BR
dc.identifier.issn23276053pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/49151-
dc.description.resumoComposites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration. When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites. Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures. The DOX/βCD composite was prepared with a double mixing method. Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days. Composite structures were evaluated by scanning electron microscopy (SEM). Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week. AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days. These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period. SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts. Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic compositept_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - FACULDADE DE ODONTOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Materials Science and Chemical Engineeringpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectb-cyclodextrinpt_BR
dc.subjectdoxycyclinept_BR
dc.subjectPolymerspt_BR
dc.subjectCompositept_BR
dc.subjectPeriodontal therapypt_BR
dc.subject.otherDoxycyclinept_BR
dc.subject.otherPolymerspt_BR
dc.subject.otherCyclodextrinspt_BR
dc.subject.otherCompomerspt_BR
dc.subject.otherPeriodonticspt_BR
dc.subject.otherOsteoblastspt_BR
dc.titleOsteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga compositept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0928493116302910?via%3Dihubpt_BR
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