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http://hdl.handle.net/1843/49151| Type: | Artigo de Periódico |
| Title: | Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite |
| Authors: | Vivianne Carvalho da Cunha Trajano Maria Esperanza Cortés Segura Ruben Dario Sinisterra Célia Regina Lanza Costa |
| Abstract: | Composites of biodegradable polymers and calcium phosphate are bioactive and flexible, and have been proposed for use in tissue engineering and bone regeneration. When associated with the broad-spectrum antibiotic doxycycline (DOX), they could favor antimicrobial action and enhance the action of osteogenic composites. Composites of polycaprolactone (PCL), poly(lactic-co-glycolic acid) (PLGA), and a bioceramic of biphasic calcium phosphate Osteosynt® (BCP) were loaded with DOX encapsulated in β-cyclodextrin (βCD) and were evaluated for effects on osteoblastic cell cultures. The DOX/βCD composite was prepared with a double mixing method. Osteoblast viability was assessed with methyl tetrazolium (MTT) assays after 1day, 7day, and 14days of composite exposure; alkaline phosphatase (AP) activity and collagen production were evaluated after 7days and 14days, and mineral nodule formation after 14days. Composite structures were evaluated by scanning electron microscopy (SEM). Osteoblasts exposed to the composite containing 25μg/mL DOX/βCD had increased cell proliferation (p<0.05) compared to control osteoblast cultures at all experimental time points, reaching a maximum in the second week. AP activity and collagen secretion levels were also elevated in osteoblasts exposed to the DOX/βCD composite (p<0.05 vs. controls) and reached a maximum after 14days. These results were corroborated by Von Kossa test results, which showed strong formation of mineralization nodules during the same time period. SEM of the composite material revealed a surface topography with pore sizes suitable for growing osteoblasts. Together, these results suggest that osteoblasts are viable, proliferative, and osteogenic in the presence of a DOX/βCD-containing BCP ceramic composite |
| Subject: | Doxycycline Polymers Cyclodextrins Compomers Periodontics Osteoblasts |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - FACULDADE DE ODONTOLOGIA |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | https://doi.org/10.1016/j.msec.2016.03.103 |
| URI: | http://hdl.handle.net/1843/49151 |
| Issue Date: | Jul-2016 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0928493116302910?via%3Dihub |
| metadata.dc.relation.ispartof: | Journal of Materials Science and Chemical Engineering |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Osteogenic activity of cyclodextrin-encapsulated doxycycline in a calcium phosphate pcl and plga composite.pdf | 928.39 kB | Adobe PDF | View/Open |
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