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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/50739
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dc.creatorJosiane de Almeidapt_BR
dc.creatorAndrea de Lima Pimentapt_BR
dc.creatorUlisses Alves Pereirapt_BR
dc.creatorLuiz Cláudio de Almeida Barbosapt_BR
dc.creatorMichel Hoogenkamppt_BR
dc.creatorSuzette van der Waalpt_BR
dc.creatorWim Crielaardpt_BR
dc.creatorWilson Tadeu Felippept_BR
dc.date.accessioned2023-03-08T14:44:13Z-
dc.date.available2023-03-08T14:44:13Z-
dc.date.issued2018-03-08-
dc.citation.volume126pt_BR
dc.citation.issue3pt_BR
dc.citation.spage214pt_BR
dc.citation.epage221pt_BR
dc.identifier.doihttps://doi.org/10.1111/eos.12411pt_BR
dc.identifier.issn1600-0722pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/50739-
dc.description.resumoThis study evaluated the inhibitory effects of lactams on Streptococcus mutans, Enterococcus faecalis, and Candida glabrata multispecies biofilm formation. γ-Alkylidene-γ-lactams 1, 2, and 3 [solubilized in 3.5% dimethyl sulfoxide (DMSO)] were tested. Glass coverslips were conditioned with either the lactams or 3.5% DMSO (control) for 1 h, inoculated with microbial cultures, and incubated for 48 h. To assess the effect of the lactams on biofilm formation, the following parameters were determined: the biofilm biomass (by both crystal violet staining and protein determination); the amount of insoluble polysaccharides of the extracellular matrix; and the number of viable and total cells [by both colony-forming unit counting and quantitative real-time PCR (qPCR)]. Data were analysed using one-way anova and post-hoc Tukey tests. Lactams 1, 2, and 3 promoted a statistically significant reduction in the amount of biofilm biomass, but only lactam 3 resulted in a statistically significant reduction in the number of attached viable E. faecalis. Both total protein content and the amount of extracellular polysaccharides decreased significantly. The effects of γ-alkylidene-γ-lactams 1, 2, and 3 on the inhibition of multispecies biofilm formation were evident by their ability to reduce the amount of protein and extracellular polysaccharides.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofEuropean Journal of Oral Sciences-
dc.rightsAcesso Restritopt_BR
dc.subjectlactamspt_BR
dc.subjectBiofilm inhibitionpt_BR
dc.subjectg-alquilydenebutenolidept_BR
dc.subject.otherBiofilmept_BR
dc.subject.otherMatriz extracelularpt_BR
dc.titleEffects of three γ-alkylidene-γ-lactams on the formation of multispecies biofilmspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/eos.12411pt_BR
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