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http://hdl.handle.net/1843/50739| Type: | Artigo de Periódico |
| Title: | Effects of three γ-alkylidene-γ-lactams on the formation of multispecies biofilms |
| Authors: | Josiane de Almeida Andrea de Lima Pimenta Ulisses Alves Pereira Luiz Cláudio de Almeida Barbosa Michel Hoogenkamp Suzette van der Waal Wim Crielaard Wilson Tadeu Felippe |
| Abstract: | This study evaluated the inhibitory effects of lactams on Streptococcus mutans, Enterococcus faecalis, and Candida glabrata multispecies biofilm formation. γ-Alkylidene-γ-lactams 1, 2, and 3 [solubilized in 3.5% dimethyl sulfoxide (DMSO)] were tested. Glass coverslips were conditioned with either the lactams or 3.5% DMSO (control) for 1 h, inoculated with microbial cultures, and incubated for 48 h. To assess the effect of the lactams on biofilm formation, the following parameters were determined: the biofilm biomass (by both crystal violet staining and protein determination); the amount of insoluble polysaccharides of the extracellular matrix; and the number of viable and total cells [by both colony-forming unit counting and quantitative real-time PCR (qPCR)]. Data were analysed using one-way anova and post-hoc Tukey tests. Lactams 1, 2, and 3 promoted a statistically significant reduction in the amount of biofilm biomass, but only lactam 3 resulted in a statistically significant reduction in the number of attached viable E. faecalis. Both total protein content and the amount of extracellular polysaccharides decreased significantly. The effects of γ-alkylidene-γ-lactams 1, 2, and 3 on the inhibition of multispecies biofilm formation were evident by their ability to reduce the amount of protein and extracellular polysaccharides. |
| Subject: | Biofilme Matriz extracelular |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/eos.12411 |
| URI: | http://hdl.handle.net/1843/50739 |
| Issue Date: | 8-Mar-2018 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/eos.12411 |
| metadata.dc.relation.ispartof: | European Journal of Oral Sciences |
| Appears in Collections: | Artigo de Periódico |
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