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http://hdl.handle.net/1843/51013| Type: | Artigo de Periódico |
| Title: | Modifying internal organization and surface morphology of siRNA lipoplexes by sodium alginate addition for efficient siRNA delivery |
| Authors: | Danielle Campiol Arruda Armando da Silva Cunha Júnior Ângelo Malachias de Souza Virginie Escriou Ismael José Gonzalez Stéphanie Finet Luis Cordova Valérie Trichet Gracielle Ferreira Andrade Céline Hoffmann Pascal Bigey Waldemar Augusto de Almeida Macedo |
| Abstract: | Vectorized small interfering RNAs (siRNAs) are widely used to induce specific mRNA degradation in the intracellular compartment of eukaryotic cells. Recently, we developed efficient cationic lipid-based siRNA vectors (siRNA lipoplexes or siLex) containing sodium alginate (Nalg-siLex) with superior efficiency and stability properties than siLex. In this study, we assessed the physicochemical and some biological properties of Nalg-siLex compared to siLex. While no significant differences in size, ζ potential and siRNA compaction were detected, the addition of sodium alginate modified the particle morphology, producing smoother and heterogeneous particles characterized by transmission electron microscopy. We also noted that Nalg-siLex have surface differences observed by X-ray photoelectron spectroscopy. These differences could arise from an internal reorganization of components induced by the addition of sodium alginate, that is indicated by Small-Angle X-ray Scattering results. Moreover, Nalg-siLex did not trigger significant hepatotoxicity nor inflammatory cytokine secretion compared to siLex. Taken together these results suggest that sodium alginate played a key role by structuring and reinforcing siRNA lipoplexes, leading to more stable and efficient delivery vector. |
| Subject: | Ácido ribonucleico Nanopartículas |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS ICX - DEPARTAMENTO DE FÍSICA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1016/j.jcis.2019.01.043 |
| URI: | http://hdl.handle.net/1843/51013 |
| Issue Date: | 2019 |
| metadata.dc.url.externa: | https://www.sciencedirect.com/science/article/pii/S0021979719300542 |
| metadata.dc.relation.ispartof: | Journal of Colloid and Interface Science |
| Appears in Collections: | Artigo de Periódico |
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